In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes a series of analytic devices. Each analytic device includes 1) a separator to separate a cellular particle from a surrounding fluid, 2) an analyzer coupled to a first outlet of the separator to analyze the surrounding fluid, and 3) at least one lysing device coupled to at least a second outlet of the separator to rupture a membrane of the cellular particle. An outlet of the lysing device is fluidly coupled to a separator of a downstream analytic device.

An air supply system including a conduit for channeling a flow of air therethrough, wherein the flow of air has microorganisms entrained therein. The system also includes an anti-microbial filter in flow communication with the flow of air. The anti-microbial filter includes a plurality of atomically sharp surface features for non-selective lysing of at least some of the microorganisms that contact the anti-microbial filter.

A device includes a microfluidic trap disposed along a microfluidic channel, the trap and channel having dimensions to create a fluid vortex within the trap to trap a particle of interest and an electrode having interdigitated electrically isolated fingers positioned in the trap to create an electric field across the trap such that the electric field causes electroporation of a molecule into the particle of interest. A further device includes an array of channels, traps and interdigitated electrically isolated fingers.

The present invention belongs to the technical field of condiment preparation, and specifically relates to an astaxanthin-containing condiment and a preparation method therefor. In this method, first a low-speed bead milling method is used to break the cell wall of the yeast, such that astaxanthin is directly emulsified in water, and the concentration of astaxanthin can reach 1043.17 mg/L, avoiding the use of emulsifiers and organic solvents; then a small amount of Angel yeast compound enzyme is added for enzymolysis, with the yield of amino nitrogen as high as 3.51% to 3.65% and the yield of solid matter as high as 47.18% to 49.22%; and finally the gelatinized porous starch solution and gelatin are sequentially added, with the encapsulation rate and drug-loading rate of the obtained astaxanthin-containing microcapsule powder being 75.62% to 88.5% and 1.55-10.42 mg/g, respectively. The microcapsule powder has bright color, and high astaxanthin stability and water solubility. The preparation method has the advantages of short time and mild conditions, which can reduce the industrial application cost of Phaffia rhodozyma.

Aspects are provided in relation to devices and systems for microorganism cell wall disruption. In this scenario, a device is provided for cell disruption of a microorganism suspension comprising (i) an inlet duct (1) of microorganisms, (ii) an annular channel (13) downstream of inlet duct (1) and in communication therewith, adapted for disruption of microorganism cells, the annular channel (13) being formed by an external part (7) and an internal part (8), the internal part being positioned inside the cavity formed by the external part (7) and (iii) an outlet duct (9) downstream of annular channel (13) and in communication therewith, for output of the ruptured microorganisms. A method is further provided that is associated with the device described above.

A system and method for mechanical processing of cells includes using a frame (102) forming an inlet channel (104), an outlet channel (106), and a processing chamber (108) fluidly connected between the inlet and outlet channels, wherein the processing chamber includes an anvil surface (112) formed on the frame. A hammer (110) mounted on the frame has a processing surface disposed in opposed relation to the anvil surface. The hammer is configured to move relative to the anvil surface. An actuator connected to the frame and operably associated with the hammer operates to move the hammer relative to the anvil surface and in close proximity to the anvil surface, wherein the hammer operates between a retracted position in which the processing surface is at a distance from the anvil surface, and an extended position in which the processing surface abuts the anvil surface.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.

A system for imploding leukemia cells of a patient includes (a) a first vessel for containing a volume of blood received from the patient, and (b) drive circuitry cooperatively coupled with at least one transducer to produce ultrasound energy that spatially decoheres and disperses throughout the volume, to implode the leukemia cells throughout the volume via absorption of the ultrasound energy by the leukemia cells. The transducer may be an immersible transducer configured to be immersed in the blood. The system may include a second vessel for containing a liquid, within which the ultrasound energy is decohered and dispersed and from which at least a portion of the ultrasound energy is transmitted to the first vessel to implode the leukemia cells.

The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening. A first portion of the partially lysed sample can be mounted on a slide and observed for atypical cells and cytologic abnormalities. A second portion of the partially lysed sample can be screened for genetic markers known to correlate with a risk of cervical cancer. Surprisingly the presence of beads in the mixture from the partial lysing process does not interfere with slide processing or cellular analysis. The method is particularly useful for cervical screening, where a combination of cytology and genetic screening present a more complete picture of cervical health. The disclosed method streamlines the diagnostic process for protocols that require both types of assays, without compromising screening accuracy.

One aspect of the invention provides an apparatus for isolating one or more subcellular components including a cell disruption reservoir that generates at least one of a phase change, a thermal change, a physical contact force, an ultrasonic frequency, an osmotic change, a pressure change, a photothermal pulse, a magnetic field, an electromagnetic field, an electric field, and an electrical pulse through the reservoir and a separation instrument configured to specifically isolate the subcellular components based on one or more parameters selected from at least one of density, charge/pH, dielectric polarization, magnetic attraction, spectral dispersion, spectral refraction, spectral diffraction, hydrophobicity, hydrophilicity, structure (presence or absence of a structural feature), function (migration), affinity or binding, and pressure.