The present algae extracts are used for biocontrol of insects. Specifically, the present extracts are obtained from Cystoseria muricatum using a polysolvent system. Once obtained, the extracts can be applied to plants to repel insects and/or to facilitate fertilization of the plants.

The present invention relates to an active ingredient comprising oligo-glucans, obtained from the cytosolic fraction of a yeast of the species Saccharomyces cerevisiae, a cosmetic composition comprising said active ingredient and cosmetic uses thereof, in particular to improve the regeneration of the skin and combat aging thereof.

An air supply system including a conduit for channeling a flow of air therethrough, wherein the flow of air has microorganisms entrained therein. The system also includes an anti-microbial filter in flow communication with the flow of air. The anti-microbial filter includes a plurality of atomically sharp surface features for non-selective lysing of at least some of the microorganisms that contact the anti-microbial filter.

A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.

Described are methods for thermal permeabilization of the biomass of microalgae of the Chlorella genus. The methods are useful in recovering the soluble fractions, including polypeptides and saccharide, of the microalgae.

The present invention relates to a method for optimising the downstream processing of a protein-rich microalgae biomass of the Chlorella genus previously prepared by fermentation in heterotrophic conditions and in the absence of light, comprising: 1) providing biomass comprising more than 50% protein by dry weight of biomass; next, at low temperature, carrying out the following steps: 2) harvesting the biomass at the end of fermentation, 3) washing and concentrating the biomass, 4) optionally, lysing the biomass, next, without low temperature stress, 5) optionally, concentrating the biomass slurry, 6) applying heat treatment, 7) drying the biomass obtained in this way in order to obtain the product, a step of adjusting the pH to 7 being applied before or after the heat treatment step 6).

The invention provides methods for extraction of fungal (e.g., yeast spp., filamentous fungal spp.) nucleic acid (e.g., DNA, RNA) from a sample (e.g., be human or veterinary clinical or research samples, agricultural samples, agricultural commodity samples, food products, or environmental samples). In some embodiments, the present invention provides enhanced nucleic acid extraction from samples comprising fungal cell(s) wherein enzymatic (e.g., lysostaphin treatment, lyticase treatment) sample treatment is performed in combination with mechanical (e.g., bead beating) sample treatment.

Method for digesting yeast, in particular brewer's yeast, for the isolation of proteins and saccharides comprising the steps: a) Providing a yeast suspension, in particular a brewer's yeast suspension; b) Physical digestion of the yeast in the yeast suspension; c) Microfiltration of the digested yeast suspension from step b); d) Ultrafiltration of the permeate of the microfiltration from step c) wherein a phase with proteins as the main solid component is separated as the retentate of the ultrafiltration; e) Treating the retentate of the microfiltration from step c) with a protease under basic conditions and subsequently separating a phase with mannan as the main solid component, in particular by filtration and/or centrifugation; f) treating the phase retained in step e) successively first at basic and then at acidic conditions and subsequently separating a phase with glucan as the main solid component, in particular by filtration and/or centrifugation.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.

A system and method for sonication of multiple samples and continuous sonication of an input fluid stream in flow-through arrangements useful for economical breakdown of particulates and organisms present in large volumes with relatively low-power sonication devices such as production of oil from algae. The system includes an electrical wave generator oscillating in the ultrasound range, a vibrating element electrically connected to the electrical wave generator, and a sonication plate that vibrates in certain modes. The sonication plate contains features for mating with sample tubes, and the sample tubes also possess complimentary mating features to those on the sonication plate. A method for sonication of multiple samples includes utilizing mating features to attach tubes to the sonication plate and energizing the sonicator to vibrate the sonication plate. The invention also relates to arrangements for continuous flow-through useful for sonicating large sample volumes.