Lysis devices, methods, and systems are disclosed including a lysis device comprising a sample vessel having an outer surface, a microchannel within the confines of the outer surface, a first port extending through the outer surface to the microchannel, and a second port extending through the outer surface to the microchannel; and an acoustic transducer bonded to the outer surface of the sample vessel to form a monolithic structure, the acoustic transducer configured to emit ultrasonic acoustic waves into and/or to induce shear forces into a blood sample within the microchannel, thereby rupturing the blood cells.

A device and method for lysing biological species present in a fluid includes implementing a rough surface against which the objects to be lysed are crushed by a shearing motion. A device for mechanically lysing biological species has a first and second wall mounted movable relative to each other, between an initial position in which the walls are separated from each other, and a lysing position in which the first wall presses against the second wall. The first and second walls also are mounted shearingly movable relative to each other in the lysing position. At least one of the first or second walls has a rough bearing surface against the other wall and has a mean surface roughness parameter Ra of between 0.2 μm and 10 μm, and preferably between 0.2 μm and 3 μm.

In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.

A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.

A device configured for lysing a sample includes a holding element configured to hold an energy-transmitting element such that the holding element lies around the held part of the energy-transmitting element and envelops the held part of the energy-transmitting element and/or that the holding element lies against the held part of the energy-transmitting element. The holding element is further configured to cause lysis with the energy-transmitting element when the holding element is immersed into the sample to be subjected to lysis or comes into contact with the sample to be subjected to lysis. A method includes inserting the energy-transmitting element into the holding element. A system configured for lysing a sample by way of ultrasound includes the device and the energy transmitting element.

A device for analyzing a biological sample which includes a separation and detection chamber into which an injection channel and a discharge channel open, a filter separating the chamber into two distinct spaces so as to define a first space into which the injection channel opens and a second space into which the discharge channel opens, the filter having a porosity suitable for the separation to be carried out, a rough bearing surface having a surface roughness parameter suitable for carrying out a mechanical lysis of the biological species present in the sample, the bearing surface being arranged in the first space, a flexible membrane arranged opposite the rough bearing surface relative to the filter and blocking an opening made through the housing.

In vitro and in vivo application of sub-atmospheric, negative pressure on growth factor starting material, such as whole blood, extracts growth factors from the platelet granules of the growth factor starting material in a non-destructive medium without activating the clotting process. The extracted growth factors are released into a growth factor composition containing blood plasma, extracellular fluid or interstitial fluid depending upon the type and location of the growth factor starting material. The growth factors have a weight of about 70-76 kDaltons and are applied in either a filtered or unfiltered state topically to the area of a surface wound to effect healing. The extracted growth factors are also injected into soft tissue, such as a torn tendon, to promote tissue growth and healing. The growth factors are released in one method from a patient's own blood. In another method the growth factors are released from a whole blood source and freeze dried by lyophilization. Then at a later date, the freeze-dried product is reconstituted by normal saline for treatment of a patient's wound, for use in a surgical procedure, or for tissue regeneration.

In one example in accordance with the present disclosure, a cell analysis system is described. The cell analysis system includes a substrate. Formed in the substrate is a feedback-controlled lysis system to rupture a cell membrane. The feed-back-controlled lysis system includes at least one lysing chamber to receive a single cell to be lysed. A lysing element of the feedback-controlled lysis system agitates the single cell and a sensor detects a state within the lysing chamber. The cell analysis system also includes a microfluidic channel formed in the substrate to 1) serially feed individual cells from a volume of cells to the feedback-controlled lysis system and 2) deliver a lysate of a ruptured cell to at least one analysis chamber. The cell analysis system also includes at least one analysis chamber formed in the substrate to process the lysate and a controller to determine when a cell membrane has ruptured.

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

The present disclosure provides functionalized powder particles and methods of forming functionalized powder particles. The functionalization is acquired through the formation of primary and/or secondary structures on a powder particle. Functionalization can be controlled to bring about changes in a broad range of physical and/or chemical properties.