Examples herein involve cell lysis with a microbead and thermal resistor. An example apparatus includes a microfluidic channel to pass a volume including a microbead and a biologic sample having nucleic acids enclosed within a cellular membrane. A first thermal resistor may be disposed within the microfluidic channel to move the biologic sample through the microfluidic channel and lyse the cellular membranes in the biologic sample to release the nucleic acids. A microfilter disposed within the microfluidic channel may filter the microbead from the biologic sample and permit the nucleic acids to pass through the filter.

A method for carbon capture and storage includes growing in water a biomass of photosynthetic microorganisms that capture carbon from a carbon source for growth; removing a portion of the biomass; and storing the removed biomass portion in an underground formation for carbon sequestration.

The present invention relates to a method for preparing a yeast protein concentrate, said method comprising the lysis of yeast cells in a suspension that was adjusted to a particular pH prior to lysis, subsequently subjecting the soluble fraction obtained from lysis to filtration to reduce the content of molecules smaller than 30 kDa, and optionally drying the solution obtained from filtration. The present invention further relates to a yeast protein concentrate obtainable by the method of the invention. The yeast protein concentrate comprises a high amount of proteins which are still folded and are therefore capable of aggregation to form a solid protein matrix upon heating. In addition, the yeast protein concentrate of the invention will be of unobtrusive taste and is therefore particularly suited for use in the preparation of food items, such as meat substitute products.

A device for breaking cells that has a reactor with a reaction chamber, an agitator, a cooling jacket, a cooling jacket inlet, a cooling jacket outlet, a sampling port, and a temperature probe insertion fitting, and a motor suitable for mounting the reactor on its top and which is operably connected with the agitator. A system for breaking cells comprising at least one device, at least one temperature probe inserted in the temperature probe insertion fitting of the device, a cooling system operably connected to the temperature probe, and an electronic control panel. The cooling system has at least one solenoid valve, each device of the system having one corresponding solenoid valve.

In one example in accordance with the present disclosure, a method is described. The method includes receiving, in a microfluidic channel, serially fed cells to be encapsulated. Each cell is individually lysed and a lysate of each cell is transported to a downstream analysis device. The lysate of an individual cell is encapsulated with an encapsulating fluid.

The present invention relates to a method for preparing a microbial cell product, said method comprising: providing an aqueous suspension comprising microbial cells; subjecting said suspension to mechanical cell disintegration at a temperature in the range of 15-35° C., preferably at a pH value in the range of 9-11, to obtain an aqueous suspension comprising disintegrated microbial cells; separating the suspension to provide an extract enriched in small cell fragments, and an extract enriched in large cell fragments; and combining at least a portion of each extract, to provide said microbial cell product. The invention further relates to a microbial cell product obtained or obtainable with said method. The invention further relates to the use of said product in foodstuffs, for example as a substitute for egg-white; in animal foodstuffs; and/or in cosmetic formulations.

Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.

A method for efficiently producing PHA comprising: inoculating PHA fermentation strains into a fermentation medium for fermentation under the condition of being capable of producing PHA through fermentation; subjecting the fermentation broth to a solid-liquid separation to obtain fermentation supernatant and thallus precipitate; breaking the cell walls of the thallus precipitate, and subjecting the wall-broken products to a plate and frame filtration to prepare PHA; pre-coating a filter cloth for the plate and frame filtration with a PHA layer; at least part of the water of the fermentation medium is PHA process wastewater. The method utilizes the PHA process wastewater as at least part of the water of the fermentation medium, and filters and separates the broken thallus with the plate and frame filtration equipment pre-coated with PHA layer to prepare PHA, thereby recycling the high-salt wastewater, reducing costs, and potentially separating PHA on a large scale for industrial production.

A cell lysis system (1) comprises a driver apparatus (2) and a cell lysis device (3) which are releasably attachable to one another. The cell lysis device (3) comprises an ultrasonic transducer (12) and a sonication chamber (11). The driver apparatus (2) drives the ultrasonic transducer (12) to output ultrasonic waves to lyse cells in a sample container (22) which is carried by the cell lysis device (3).

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.