Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

The invention relates to novel algae of the genus Coccomyxa, in particular the algae of a new species called C-IR3-4C, and their use for capturing metals from aqueous media, and in particular from radioactive media.

The invention concerns a strain of Shizochytrium mangrovei filed on 22 Nov. 2012 with the CNCM as number 1-4702 having the ability to produce a high quantity of docosahexaenoic acid (or DHA) and palmitic acid, the methods for producing the corresponding biomass containing said lipid compounds of interest, and the biomass containing the products and compositions prepared from this strain.

Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalgae take up and store wastewater P as polyphosphate as they grow in a partially submerged revolving biofilm, which may then be harvested and dried for use as fertilizer in lieu of mined phosphate rock. Disclosed herein are methods for isolating and characterizing microalgae strains from active RAB systems. Strains were identified by microscopy and 16S/18S ribosomal DNA sequencing, cryopreserved, and screened for elevated P content (as polyphosphate). Seven isolated strains possessed at least 50% more polyphosphate by cell dry weight than a microalgae consortium from a RAB system, with the top strain accumulating nearly threefold more polyphosphate. Isolated P-hyperaccumulating microalgae have broad applications in resource recovery from various waste streams, including improving P removal from wastewater.

The present application relates to a novel Schizochytrium sp. strain easy to extract oil in a cell and a method for producing oil containing omega3 using thereof, and the novel Thraustochytrid series microalgae of the present invention have a high fat content in biomass and have a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid among them, and it is very easy to extract biomass produced by itself or by culture and fermentation and fat components including unsaturated fatty acids from biomass. Accordingly, the microalgae, the biomass dried product and bio-oil produced therefrom can be usefully used as a composition for feed or a composition for food, or the like.

The invention provides methods of transforming photosynthetic organisms, such as green algae. The methods involve methylating one or more DNA fragments of a DNA construct and transforming the one or more fragments into the photosynthetic organism. The DNA fragments can be the product of a DNA amplification procedure, such as PCR or a PCR-like procedure. In one embodiment the one or more fragments of DNA that comprise a DNA construct are dam methylated prior to being transformed into the photosynthetic organism.

The present invention relates to a process for producing enriched algal biomass having high lipid productivity. More particularly, the present invention provides a process for obtaining an enriched biomass with omega-3 fatty acids by using a microalgal strain Schizochytrium MTCC 5890 in a unique media composition and substrate residual band in a steady state continuous fermentation. The process of the present invention results in high biomass and lipid productivity.

The present invention discloses a pre-stress treatment method for reducing a mortality rate of Haematococcus pluvialis. The method includes the following steps: after the Haematococcus pluvialis is subjected to a logarithmic-growth amplification stage to reach a specific biomass and before the Haematococcus pluvialls enters into an accumulation stage of astaxanthin, performing prestress treatment on the Haematococcus pluvialis, where the prestress treatment indicates adjusting a culture system to include characteristic peaks with wavelength ranges of 430-490 nm and 620-700 nm as spectra parameters. In the present invention, ecological factors in the culture system of the haematococcus pluvialis are adjusted, so that more than 90% of cells therein can detach from flagella in a short time, the walls of the cells are thickened, the cells enter into an ideal immobile cell state in which the astaxanthin can be accumulated, and the mortality rate of the cells is less than 3%, so as to reduce the mortality rate of the cells at an initial stage in which the Haematococcus pluvialis stresses to accumulate the astaxanthin. This reduces the generation of organic matters in an algal liquid, and realizes the purpose of reducing pollution, shortens the accumulation period of the astaxanthin in the Haematococcus pluvialis, and improve the productivity, the quality and yield of the product and the production stability.

The invention relates to isolation, purification, and in vitro cultivation of Symbiodinium algae symbiotic with soft corals. The deposit information for Symbiodinium sp. SY-1 is as follows: Depository Name: China General Microbiological Culture Collection Center (CGMCC); Depository Address: Institute of Microbiology, Chinese Academy of Sciences, Building 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China; Deposit Date: Jun. 7, 2023; Deposit Number: CGMCC No. 40681; Taxonomy: Symbiodinium sp.

Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.