Patent classifications
C12N1/125
Biomass of the microalgae Schizochytrium mangrovei and method for preparing same
The invention concerns a strain of Schizochytrium mangrovei, filed on 22 Nov. 2012 with the CNCM as number I-4702, having the ability to produce a high quantity of docosahexaenoic acid (or DHA) and palmitic acid, the methods for producing the corresponding biomass containing said lipid compounds of interest, and the biomass containing the products and compositions prepared from this strain.
Schizochytrium limacinum strain, building method therefor and application thereof
Provided is a Schizochytrium limacinum strain, a building method therefor and an application thereof. The strain disclosed is classified and named as Schizochytrium sp. HX-RS, and the preservation number is CCTCC NO: M2017046. An acyltransferase functional domain originating from Shewanella PKS enzyme is adopted instead of an acyltransferase functional domain originating from Schizochytrium sp. PKS enzyme, and the strain is obtained by performing flat panel screening and acclimation screening with a high rotation seed and a low temperature.
DIRECTED EVOLUTION USING TURBIDOSTAT FOR INCREASED SPECIFIC GROWTH RATE AND REDUCED LIGHT-HARVESTING ANTENNA SIZE OF PHOTOSYNTHETIC MICROORGANISMS FOR INCREASED PHOTOSYNTHETIC EFFICIENCY
Methods are provided to select strains of photosynthetic microorganisms for enhanced photosynthetic efficiency or biomass accumulation. Strains are mutagenized, and then grown under high light in a turbidostat. Microorganisms created by this process are also described, as well as methods of using such isolated microorganisms for biomass production.
LABYRINTHULID MICROORGANISM CAPABLE OF PRODUCING MICROBIAL OIL, MICROBIAL OIL, METHODS FOR PRODUCING SAID MICROORGANISM AND FOR PRODUCING SAID MICROBIAL OIL, AND USES OF SAID MICROORGANISM AND SAID MICROBIAL OIL
A method for producing a microbial oil includes steps of: genetically modifying a labyrinthulid by disrupting and/or silencing a gene, or by transforming another gene in addition to the disruption and/or gene silencing of the gene, and culturing the labyrinthulid, such that a fatty acid composition accumulated in the labyrinthulid comprises an increased EPA content; and collecting the microbial oil having the increased EPA content from the labyrinthulid. The labyrinthulid before the modification is selected from (A) a labyrinthulid belonging to the genus Parietichytrium or genus Schizochytrium and having very weak or no activity of producing PUFAs via a PUFA-PKS pathway; and (B) a labyrinthulid belonging to the genus Thraustochytrium in which a host PUFA-PKS gene is disrupted or silenced to a very weak level. The increased EPA content is preferably not less than 11.5% of a total fatty acid composition.
METHODS FOR OVERPRODUCING PROTOPORPHYRIN IX IN ALGAE AND COMPOSITIONS THEREFROM
Provided herein are compositions and processes for producing compositions from an algae that overproduces protoporphyrin IX (PPIX). Also provided are methods of growing PPIX overproducing algae, methods of isolating PPIX-containing portions from algae cultures and compositions and methods of making food products with PPIX produced by algae. Provided herein are strains and methods to select strains that overproduce PPIX. Also provided are compositions, including edible compositions that include PPIX produced from algae. Specifically, the algal strain over-producing PPIX is an engineered strain of The algal biomass may be used for preparing meat analogues wherein the PPIX compound imparts a meat-like colour and flavour.
Methods for producing biomass rich in DHA, palmitic acid and protein using a eukaryotic microorganism
Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.
METHODS FOR PRODUCING BIOMASS RICH IN DHA, PALMITIC ACID AND PROTEIN USING A EUKARYOTIC MICROORGANISM
Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.
BIOMASS GROWTH COMPOSITION CONTAINING HAEMATOCOCCUS SP. STRAIN
The invention is directed to Haematococcus sp. KAU-01 as well as to a culture medium for Haematococcus sp. KAU-01, and to methods for using this strain to process environmental pollutants such as gases generated by coal-fired plants.
Microalgae adapted for heterotrophic culture conditions
The present invention provides methods for making microalgal strains with improved properties relative to the strains from which they are derived. In illustrative embodiments, the methods are performed to produce microalgal strains adapted for use in the industrial production of microalgae-derived biomass products, including but not limited to triglycerides and fatty acids. Also provided are microalgal strains, which can be obtained using the methods described herein, as wells microalgal-derived biomass products, which can be produced from such microalgal strains.
Cyanobacterial hosts and methods for producing chemicals
The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.