Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption.

The mutant strain of the alga Nannochloropsis is provided. A method for producing the mutant strain of the alga Nannochloropsis is also provided. Use of compounds produced from the mutant strain of the alga Nannochloropsis is further provided.

This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria, that have a reduced amount of a light harvesting protein (LHP) and contain one or more introduced or overexpressed polynucleotides encoding one or more enzymes associated with lipid biosynthesis, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.

Docosahexaenoic acid-containing oil containing docosahexaenoic acid in a concentration of 40 wt. % or more of the total weight of fatty acids in the oil, and having an endothermic peak temperature determined by differential scanning calorimetry (DSC) of 15 C. or lower; a biomass including the same; and a method for producing docosahexaenoic acid-containing oil including obtaining a biomass by culturing microorganisms of the genus Aurantiochytrium capable of producing this docosahexaenoic acid-containing oil, recovering the biomass after culture, and extracting the oil from the biomass after recovery.

The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.

Provided herein are methods for obtaining purified recombinant osteopontin (rOPN) from cultures of transgenic microalgae, as well as nutritional supplements from such cultures.

The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved. The bacteria provided by the invention, the six gene fragments, the protein encoded by these six gene fragments, the vector, the cell or the organism containing these six gene fragments all have important application values.

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption.

The invention discloses the use of the Schizochytrium limacinum and its preparation in improving the quality and yield of animal product. The deposit number of Schizochytrium limacinum in the present invention is CGMCC No. 13746 in the China General Microbiological Culture Collection Center. The Schizochytrium limacinum powder produced by the Schizochytrium limacinum may increase the DHA content in an animal product, reduce the cholesterol content in an animal product, and also improve the egg production performance of poultry. This animal product with high DHA content from natural sources is organic, safe, stable, and easy to be absorbed. It may be used as a safer and effective way for people to ingest natural DHA, and it may also cater to and meet consumer needs. Thus, Schizochytrium limacinum and Schizochytrium limacinum powder of the present application have a wide range of application in the field of general food and livestock breeding.

The present disclosure relates to strains of Thraustochyrium genus, including a high content of polyunsaturated fatty acids, and a method of producing a biomass using the same. According to the novel CJM01 microalgae of Thraustochyrium genus of the present disclosure, the content of lipids in the biomass and the content of unsaturated fatty acid such as docosahexaenoic acid in the biomass are high, so that the microalgae itself, a biomass produced by the culturing and fermentation of microalgae, a condensate of the biomass, and a dried product of the biomass are very useful as a feed composition.