Provided herein, inter alia, are methods, host cells, and vectors for producing 3-hydroxypropionate (3-HP). In some embodiments, the host cells include a recombinant polynucleotide encoding an oxaloacetate decarboxylase (OAADC) and a polynucleotide encoding a 3-hydroxypropionate dehydrogenase (3-HPDH). In some embodiments, the methods include culturing said host cell(s) in a culture medium comprising a substrate under conditions suitable for the recombinant host cell to convert the substrate to 3-HP. Expression of the OAADC and the 3-HPDH results in increased production of 3-HP, as compared to production by a host cell lacking expression of the OAADC and the 3-HPDH.

The present disclosure concerns using an inactivated yeast product made from a yeast host cell to increase the yield of a fermentation product from a fermenting yeast host cell. The inactivated yeast extract can be formulated as a liquefaction or fermentation additive and can be used to improve the yield of a fermented product such as ethanol.

The present invention relates to a process for valorization of low-cost alkane rich feedstock. More specifically, the present invention relates to the selective conversion of alkane rich kerosene to value-added products like mono/dicarboxylic acid, fatty acids and biosurfactants using mutant yeast strain and a heterogenous nano-catalyst. The present invention also provides a mutant yeast strain for selective conversion of alkane rich refinery stream from a substrate containing hydrocarbons. The mutant yeast strain of the present invention is able to consume the sulfur content in the feed and results in the desulfurization of the alkane rich feedstock.

The present invention relates to a process for valorization of low-cost alkane rich feedstock. More specifically, the present invention relates to the selective conversion of alkane rich kerosene to value-added products like mono/dicarboxylic acid, fatty acids and biosurfactants using mutant yeast strain and a heterogenous nano-catalyst. The present invention also provides a mutant yeast strain for selective conversion of alkane rich refinery stream from a substrate containing hydrocarbons. The mutant yeast strain of the present invention is able to consume the sulfur content in the feed and results in the desulfurization of the alkane rich feedstock.

Processes and systems for biologically producing hydrogen gas from organic waste, including food waste. Such a process includes biologically producing hydrogen gas from organic waste by anaerobic fermentation of the organic waste with at least one strain of yeast.

Metabolites produced by a microorganism using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and more particularly using oxaloacetate. There is indeed a need in the art for transformed, in particular recombinant, microorganisms having at least an increased ability to produce oxaloacetate, pyruvate and/or acetyl-CoA, and in particular oxaloacetate, thus allowing an increased capacity to produce metabolites produced using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and in particular amino acids and their derivatives thereof, fatty acids, derivatives from the mevalonate pathway (in particular farnesyl, squalene, lanosterol, cholesterol and derivatives, and dolichols), flavonoides and/or polyketides. The solution proposed is the use of a genetically modified yeast comprising many modifications as described in the present text.

Metabolites produced by a microorganism using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and more particularly using oxaloacetate. There is indeed a need in the art for transformed, in particular recombinant, microorganisms having at least an increased ability to produce oxaloacetate, pyruvate and/or acetyl-CoA, and in particular oxaloacetate, thus allowing an increased capacity to produce metabolites produced using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and in particular amino acids and their derivatives thereof, fatty acids, derivatives from the mevalonate pathway (in particular farnesyl, squalene, lanosterol, cholesterol and derivatives, and dolichols), flavonoides and/or polyketides. The solution proposed is the use of a genetically modified yeast comprising many modifications as described in the present text.

The disclosure relates to the production of lipids by microbes utilizing novel post-fermentation industrial feedstocks. The post-fermentation industrial feedstocks comprise one or more inhibitory compounds, which traditionally have made the post-fermentation media unsuitable for utilization as a feedstock for microbial lipid production. In aspects, the disclosure provides oleaginous yeast capable of utilizing these post-fermentation industrial waste streams as a novel feedstock, methods of producing lipids and microbial oils utilizing these microbes and feedstock, and novel compositions produced from the methods.

The disclosure relates to the production of lipids by microbes utilizing novel post-fermentation industrial feedstocks. The post-fermentation industrial feedstocks comprise one or more inhibitory compounds, which traditionally have made the post-fermentation media unsuitable for utilization as a feedstock for microbial lipid production. In aspects, the disclosure provides oleaginous yeast capable of utilizing these post-fermentation industrial waste streams as a novel feedstock, methods of producing lipids and microbial oils utilizing these microbes and feedstock, and novel compositions produced from the methods.

According to an embodiment, there are provided a screening culture medium that enables a convenient and quick examination for the presence of Candida auris, and a screening method. According to at least one embodiment, the screening culture medium contains an enzyme substrate, in which the screening culture medium is used for screening Candida auris based on an ability to degrade the enzyme substrate, the enzyme substrate including raffinose and xylose. According to another embodiment, the screening method uses the screening culture medium.