The present invention relates to Nelumbo nucifera callus having an increased content of gallic acid or an extract thereof and to a method for preparing the same. The Nelumbo nucifera callus extract according to the present invention has an excellent whitening effect by containing a large amount of gallic acid, and thus can be advantageously used as a cosmetic composition.

The invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused.

The present disclosure provides a planar microelectronic human blood brain barrier stack used to model drug effects and transport across the brain capillary endothelial barrier to neurons. In one embodiment the stack is comprised of a carrier substrate, electrode arrays, astrocytes, extracellular matrix and brain capillary endothelial cells.

The invention provides a callus induction medium for blueberry tissue culture, taking woody plant medium (WPM) as a basic medium, and including: 0.5-5.0 mg/L forchlorfenuron (CPPU) and 0.1-0.4 mg/L 2-isopentenyladenine (2-ip). The present invention also provides a callus culture method for blueberry, including inoculating the blueberry explant into the above callus induction medium to conduct induction culture in order to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, one can conduct continuous differentiation, and the effect is better on multiple varieties.

Example embodiments in accordance with the present disclosure are directed to methods comprising contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation, and culturing the plant part under growth conditions to enhance PCM formation.

The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.

The present invention relates to a method for mass-production of viniferin using stevioside from cell culture of grape tree tissue. Viniferin is known to be effective for protection of liver, anticancer, antioxidant, and skin whitening, have an effect of inhibiting oxidation of low-density lipoprotein and high-density lipoprotein and inhibiting the proliferation and migration of vascular smooth muscle cells. Therefore, the present invention is very useful for the mass production of viniferin among the useful substances (stilbene compounds) from a callus derived from the anther tissue of the grape plant, which is very important for the related industries.

The present invention relates to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives, the method comprising the steps of: (a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives; and (b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a). The present invention further relates to a plant suspension cell culture, wherein the cells are obtained from a plant selected from the family Euphorbiaceae, and wherein the plant cells produce Ingenol and/or one or more Ingenol ester and/or one or more Tiglian-3-one derivatives.

The present invention provides a method for producing a composition for culturing animal cells, wherein the method includes: (1) a step in which an algae is mixed with a solid acid catalyst and an algae extract is obtained by heating; and (2) a step in which the algae extract is added to a medium for culturing animal cells and the concentration of the algae extract is adjusted. The present invention also provides a recycling/culturing method for algae and animal cells including: (i) a step in which a waste liquid (a first waste liquid) previously used to culture animal cells is used to culture algae; (ii) a step in which the algae is collected, mixed with a solid catalyst, and heated, and an algae extract is thereby obtained; (iii) a step in which the algae extract is added to the waste liquid (a second waste liquid) previously used to culture algae in (i), the concentration of the algae extract is adjusted, and a composition for culturing animal cells is produced; and (iv) a step in which animal cells are cultured using the composition for culturing animal cells.

The present application describes a large scale process for the in vitro production of an olive cell culture.

The application further describes a composition in a form of a powder comprising olive fruit/leaf cells grown in vitro and a method of treating metabolic syndrome disorders, such as, high cholesterol level, comprising administering an effective amount of the composition. The cell line callus culture of olive cells manufactured according to the process of the invention includes high level of hydroxytyrosoll, tyrosol, oleoropein and verbascoside.