A composite shell particle including a composite shell layer is provided. The composite shell layer is a hollow shell, wherein the composite shell layer includes a porous biological layer and a metallic layer. The porous biological layer is composed of an organic substance including a cell wall or a cell membrane of a bacteria or algae. The metallic layer is crosslinked with the porous biological layer to form the composite shell layer. The metallic layer includes at least one metal selected from the group consisting of iron, molybdenum, tungsten, manganese, zirconium, cobalt, nickel, copper, zinc, and calcium, and/or includes at least one selected form the group consisting of metal chelates, metal oxides, metal sulfides, metal chlorides, metal selenides, metal acid salt compounds, and metal carbonate compounds. A method of manufacturing the composite shell particle, and a biological material including the composite shell particle and the applications thereof are also provided.

Plant cell cultures as well as related methods, systems, and compositions for increasing the frequency and efficiency of plant genome editing are provided. Various plant cell growth conditions and/or treatments where such increases in gene editing frequencies are obtained are disclosed.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Plant cell cultures as well as related methods, systems, and compositions for increasing the frequency and efficiency of plant genome editing are provided. Various plant cell growth conditions and/or treatments where such increases in gene editing frequencies are obtained are disclosed.

The present invention belongs to the field of plant biotechnology engineering, and particularly relates to a method for improving flavonoid phenylpropanoid compounds in a Saussurea involucrata cell culture. In a medium used in the method, a phosphorus concentration is 0.5-3 mmol/L, a NO.sub.3.sup.− ion concentration is 20-35 mmol/L, a NH.sub.4.sup.+ ion concentration is 0-30 mmol/L, a Ca.sup.2+ ion concentration is 0.5-3 mmol/L, a Mg.sup.2+ ion concentration is 0.2-1.5 mmol/L, and a boron ion concentration is 0.02-0.1 mmol/L; an inducer or precursor or bypass metabolism inhibitor may be added to the medium. Finally, the content of total flavonoids and the contents of rutin, chlorogenic acid, syringin and 1,5-dicaffeoylquinic acid in the Saussurea involucrata cell culture are significantly improved.

Embodiments described herein provide for multi-media structures 100 with growth enhancement additives for multiple stages of growth of an organism such as a plant, fungus or bacteria, including the production of individual media structures and multi-media structures 100 for multi-stage growth. Methods for the production of individual media structure and multi-media structures 100 with growth enhancement additives are provided. Methods for using multi-media structures 100 to grow an organism through multiple stages of growth such as root production, vegetative growth and flowering are also provided.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite selected from aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI) and N-acetylputrescinium (NAP) below an inhibitory concentration in the cell culture medium for the at least one metabolite.