Certain exemplary embodiments are directed to a biologically active composition of matter (and uses thereof) configured for targeted delivery of biotin to mitochondria, the composition comprising a first D-biotin conjugated to a water-soluble, cell-permeable, peptide sequence, wherein the peptide sequence is selected from a polypeptide group with an alternating aromatic-cationic motif.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

The present disclosure pertains to compositions comprising aflibercept and methods for producing such compositions in chemically defined media and using anion exchange chromatography to produce aflibercept with a specific glycosylation profile. In particular, these methods produce aflibercept with a glycosylation profile with specific ranges of % total fucosylated glycans, total sialylated glycans, mannose-5, and galactosylated glycans.

The present disclosure pertains to methods for preparing a prefilled syringe and methods for preparing an auto injector prefilled syringe comprising aflibercept and methods for producing such aflibercept in chemically defined media.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

A compound additive having a biological activation function. The compound additive contains water or phosphate buffer, and multiple proteins and various factors dissolved therein. The compound additive can be added into a culture medium for cell cultivation, and can also be directly used or added into a skin repair product or a cosmetic product so as to achieve certain skin repair and cosmetic effects.

Non-naturally occurring thermogenic adipocytes are provided. The cells have a distinctive molecular signature, and can be distinguished from predecessor cells such as adipose-derived stem cells as well as other naturally occurring and induced thermogenic cells. Differentiation media that can induce differentiation of ADSC and white adipocytes into thermogenic adipocytes is also provided. Methods of making thermogenic adipocytes, thermogenic adipocytes made according the disclosed methods as well as conditioned media made according a method of incubating the cells in a tissue culture media are also provided. Compositions including thermogenic adipocytes, conditioned media, secreted factor(s), active agents that increase the number or activity of thermogenic adipocytes, or a combination thereof are also disclosed and can be used to treat a variety of diseases and conditions, particularly obesity and metabolic disorders.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.