The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures.

Provided are a cell growth method including serum-free culture of somatic cells sowed in a cell growth medium containing a culture supernatant of dental pulp stem cells, wherein the somatic cells exclude physically or physiologically defected somatic cells, is a novel cell growth method for serum-free culture of somatic cells.

The invention relates to a method and composition for the cultivation, expansion and production of extracellular vesicles, and the preservation and/or pre-treatment of cells, wherein said composition is a medium or media supplement or a solution comprising pterostilbene or the derivatives thereof, and/or a migration inhibitory factor antagonist and optionally a bivalent cation.

The invention relates to a method of culturing mammalian cells expressing a heterologous protein in a perfusion cell culture comprising increasing the potassium concentration and decreasing the molar ratio of sodium to potassium to reduce wasteful cell bleed and to increase protein production. The invention further relates to a serum-free perfusion medium comprising a high potassium ion concentration and a low molar ratio of sodium to potassium and to the use of this medium for use in culturing cells in a perfusion culture during production phase or for reducing the cell bleed volume during production phase.

A stem cell agent for anti-hypoxia injury and its preparation method, and its application in the drugs for the therapy of acute myocardial infarction. The preparation method for the stem cell agent for anti-hypoxia injury comprises mixed stem cells and a liquid of lentivirus with Fstl1 over-expression in a medium to obtain the stem cell agent for anti-hypoxia injury. The Fstl1 modified MSCs provided by the present invention have anti-hypoxia injury capability, can improve transplant survival rate, and can improve the cardiac function after infarction by means of direct injection into the cardiac muscle.

The invention provides a method for inducing adenohypophysis or precursor tissue thereof in vitro from human pluripotent stem cells. The method includes culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signal pathway to obtain a human cell aggregate containing a hypothalamus neuroepithelial tissue and a surface ectoderm, and further culturing the obtained human cell aggregate containing the hypothalamus neuroepithelial tissue and the surface ectoderm in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signal pathway to induce formation of hypophysial placode and/or Rathke's pouch in the surface ectoderm, thus obtaining a human cell aggregate containing 1) hypothalamus neuroepithelial tissue, and 2) hypophysial placode and/or Rathke's pouch.

This application describes liver stem cells (LSC), and differentiated hepatocytes, cholangiocytes, and 3D cellular structures derived therefrom. Methods for producing LSC and mature, differentiated hepatocytes and cholangiocytes in culture are provided. Also provided are cell culture systems and cell culture media for producing a homogenous population of liver stem cells that remain in an undifferentiated state over multiple passages in culture. The LSC and methods are useful for producing homogenous populations of hepatocytes and cholangiocytes for downstream applications. The LSC can be transplanted into subjects to treat liver diseases.

The present invention relates to methods of modulating (e.g., reducing) the mannose content, particularly high-mannose content of recombinant glycoproteins.

Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

The present invention relates to: a cosmetic composition for improving the condition of the skin, containing, as active ingredients, a cell culture medium, an epidermal growth factor and a bovine serum albumin; a method for improving the condition of the skin by using the same; and a method for preventing or treating skin diseases. According to the present invention, the cosmetic composition can exhibit excellent wrinkle-reducing and wound-healing effects even when only a small amount of EGF is used, and thus can be useful in improving the condition of the skin or in preventing or treating skin diseases.