A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

Systems and methods for the continuous production of digestible scaffolds for three-dimensional cell growth applications are provided. Systems for producing cell culture scaffolds include a housing and a plurality of modular components disposed in a serial arrangement within the housing, the modular components connected through a plurality of microfluidic flow channels. The modular components may comprise an emulsifier, a coating reactor, and a separator. Optionally, systems may comprise a porous membrane. Methods include cross-linking of polymer solutions into gels of discrete shapes; binding a layer of cell growth media to the gels; and drying or dehydrating the gels to form an aerogel functionalized for use as cell growth media.

A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

The invention relates to devices for the culture of cellular aggregates comprising a three-dimensional network of interconnected vessels of a biocompatible liquid- and gas-permeable photo polymerised material, wherein the three-dimensional network is connected to a plurality of inlets and a plurality of outlets for the delivery of liquids, and overlayed with a secondary hydrogel network wherein the cellular aggregates are embedded. Herein defined vessels within the three-dimensional network are blocked, thereby defining at least one spatially segregated network within and structurally in contact with the three-dimensional network, wherein the at least one spatially segregated network is connected to a separate inlet and a separate outlet, allowing the supply of a liquid to a subregion within the three-dimensional network.

The invention relates to a carrying device for beverage cans which allows the manual carrying of beverage cans grouped together in the form of a pack, which device comprises a body devoid of side walls, having on its surface at least one opening defining a contour with proportions which allow the tight passage therethrough of a beverage can, the contour of said opening having a plurality of tabs that can be folded or bent in relation to the body itself and extending into the same opening, said tabs having a distribution according to at least one sequence, said sequence comprising two contiguous tabs followed by a gap followed by another tab followed by another gap.

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.

The present invention relates to a method of preparing magnetic oocytes and/or embryos by means of nanoparticles for assisted reproduction techniques and to the use in assisted reproduction techniques of non-human oocytes and/or embryos prepared with said method. Said method comprises: a step in which magnetic nanoparticles are conjugated with oviduct recombinant protein, OVGP1r; a step in which it is verified that the nanoparticle-OVGP1r conjugation attaches to the ZP of oocytes/embryos after being incubated together; and a step in which the number of oocytes/embryos having nanoparticles attached to them distributed around the ZP without being endocytosed is verified; and it is assessed if the amount of magnetic nanoparticles attached to the ZP of oocytes/embryos is enough to be attracted by a magnetic field.

Methods for producing megakaryocytic progenitors (preMKs) and megakaryocytes (MKs) from stem cells are provided. The present disclosure further provides compositions comprising preMKs and MKs and their lysates, and also methods of use of preMKs, MKs, their lysates and compositions thereof.

The present invention is directed to a method of producing extracellular vesicles compositions from conditioned culture medium (CCM) compositions. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble (CCM) and non-soluble fractions. Extracellular vesicles are derived from the soluble fraction (the CCM) produced by the cells cultured under hypoxic conditions and may be used in a variety of medical and therapeutic applications.

Described herein are methods and compositions for use in expanding alveolar epithelial cells. The methods may include the use of three-dimensional substrates and improved techniques for expansion of the cells. The improved composition for culturing alveolar epithelial cells may include at least one or more of the following: a TGF- pathway inhibitor; a Wnt pathway activator; a ROCK inhibitor; an epidermal growth factor (EGF); a keratinocyte growth factor (KGF); and a fetal bovine serum.