The present disclosure provides feeder hydrogel particles that can function to support the growth, proliferation, and/or activation of a target cell in culture. The present disclosure also provides methods of culturing target cells with feeder hydrogel particles.

A body cube for culturing tissue that includes: an organ chip holder; and a body barrier chip and a first body organ chip disposed in the organ chip holder, the first body organ chip including a first cell culture chamber that receives cell culture medium and produces a first tissue in the first cell culture chamber, such that the organ chip holder receives cell culture medium and communicates the cell culture medium to the first cell culture chamber of the first body organ chip in response to rotation of the organ chip holder.

The present disclosure provides materials and methods identifying, selecting, and characterizing cells that express and secrete non-Fc containing biomolecules.

Provided is a cell culture microcarrier capable of suppressing the aggregation of a microcarrier due to cell clumps and enhancing the efficiency of culturing cells. The cell culture microcarrier according to the present invention includes a base particle and a coating layer coating the outer surface of the base particle, and has a specific gravity of 1.10 g/cm.sup.3 or more.

Provided is a microcarrier for cell culture in which adhesion between microcarriers due to a cell mass can be suppressed. The microcarrier for cell culture according to the present invention includes a base particle and a coating layer covering an outer surface of the base particle, and the coating layer includes a resin having a polyvinyl alcohol derivative skeleton or a poly(meth)acrylic acid ester skeleton and having a peptide moiety, and the microcarrier has an average particle size of 300 ?m or more and a CV value of particle size of 10% or less.

A method for non-enzymatic 3D culture and amplification of mesenchymal stem cells (MSCs) includes the followings steps: preparing PLGA porous microspheres; preparing a PLGA-PEG-PLGA thermosensitive coating microcarrier; culturing and amplifying MSCs; and performing non-enzymatic separation of MSCs, including reducing a culture temperature to below a critical phase transition temperature, and centrifuging a culture medium to collect stem cells. The present invention adopts the method for non-enzymatic 3D culture and amplification of MSCs, wherein the PLGA porous microspheres are used as a cell culture microcarrier scaffold and the thermosensitive hydrogel PLGA-PEG-PLGA is coated on surfaces of such microspheres, without needing additional enzymolysis process, thus efficiently amplifying the stem cells.

Methods of making a spheroid are provided whereby a suspension is first produced including one or more biologically-relevant materials dispersed within a biocompatible medium. A droplet of the suspension is then bioprinted into a salt solution by bringing the droplet into contact with a surface of the salt solution in a controlled manner to reproducibly yield a spheroid containing a desired size and a desired amount of biologically-relevant materials.

Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., >70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.

Beads containing a polymer containing uronic acid units having mercapto groups, in which the mercapto groups partly or entirely form disulfide bonds, are useful for encapsulating cells and microorganisms.

An object of the present invention is to provide a preservative solution for human stem cells, a human stem cell suspension, and a method for preserving human stem cells, which enable preservation of human stem cells at a high survival rate for cells. According to the present invention, a preservative solution for human stem cells is provided. The preservative solution includes at least human serum, in which a volume ratio of human serum with respect to the entire preservative solution is 0.70 or more, and the human stem cells are preserved at a temperature higher than 0 C.