The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, such products may include fat replicas, which may improve taste, appearance, etc. In some cases, a fat replica can be formed by forming an emulsion of fat and a non-human blood plasma, then causing the blood plasma to crosslink and/or clot, e.g., forming a hydrogel containing the fat emulsion. In some cases, the fat replica may be used to make a cultivated meat product, e.g., by combining with muscle replicas, lysate of non-human red blood cells, etc. Other embodiments are generally directed to methods of making or using such fat replicas, the microcarriers, or the cultivated meat products, kits involving these, or the like.

The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, muscle and/or fat cells can be grown on microcarriers or other scaffolds, for example, in a bioreactor or other in vitro cell culture system. The microcarriers or other scaffolds can comprise materials such as fibrin. The fibrin may be formed into hydrogels or other articles, which may be edible in some cases. The microcarriers may also contain grooves or other structures in some instances. In certain embodiments, the microcarriers may be present within the final product, e.g., in a cultivated meat product. Other embodiments are generally directed to methods of making or using microcarriers or cultivated meat products, kits involving these, or the like.

The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, the whole blood of a non-human animal is separated into various components (e.g., concentrated red blood cells, blood plasma, etc.). Some embodiments are generally directed toward incorporating coloring or redness into a product. For example, colorants may be added to a cultivated meat product to improve its color. The colorant may comprise a lysate of non-human red blood cells, e.g., containing hemoglobin. In some embodiments, the lysate may be obtained from blood withdrawn from living animal donors. Other embodiments yet are generally directed toward the composition and method of use of the lysate, cultivated meat products, kits involving these, or the like.

A method of coating a surface with nanoparticles for biological analysis of cells that includes the steps of cleaning the surface with an oxidizing acid, treating the surface with an organosilane, coating the surface with nanoparticles, and then growing cells on the surface coated with the nanoparticles. The surface may be a glass surface, a silica-based surface, a plastic-based surface or a polymer-based surface. The nanoparticles may be gold-based nanomaterials.

A composition comprising a cell population wherein the population is suitable for transplantation into a subject in need thereof, and characterized by differences of expression levels of a plurality of genes. Further, methods and kits for identifying a cell population suitable for transplantation into a subject in need thereof.

A large circulating fluidized bed cell bioreactor and a method for culturing animal cells. The reactor comprises an agitator tank body (01), an agitator tank base (50), a water inlet silicone hose (06), a backflow silicone hose (08), a cell culture tank (10), a culture tank base (40), a backflow pipe (15), a water inlet pipe (12), an agitator (20), a reactor tray (30), and a tube support plate (60). By means of cooperation among the above components, defects and shortcomings of fluidized bed cell bioreactors in the prior art including proneness to microbial contamination, a low level of dissolved oxygen, and a low working volume, are effectively resolved, advantageous in the popularization and application of this technology.

Disclosed herein are methods of producing chimeric antigen receptor (CAR) T cells using substrates, such as artificial antigen presenting cells, containing on a surface a a heparin binding domain (HBD), anti-CD3 single chain antibodies, anti-CD28 single chain antibodies (scFv), and optionally anti-41BBL antibodies. Anti-CD3 and Anti-CD28 scFvs bind and activate expanding T cells ex vivo, while the Heparin Binding Domain binds the viral vector, thereby bringing the T cells into close proximity with virus for effective gene transfer. This is a less costly, renewable, modifiable, and efficacious alternative to coated beads and RetroNectin? for gene transfer.

The present invention is directed to methods for forming microparticles useful for cell seeding and for conjugating protein to the surface of the microparticles. The method comprises co-injecting an organic solution of PLGA or other polymer with an aqueous solution into a flow focusing tube.

A spheroid tissue microarray comprises an array of tissue spheroids embedded within a porous mold. The product may be impregnated with a wax or resin and sectioned, and contains spheroids which are precisely located in a regular geometric grid. A method of manufacturing a spheroid tissue microarray comprises the steps of: forming a mold of porous material from liquid mold material in a casting mold, and allowing the liquid mold material to set; removing the porous mold from the casting mold; topping up the porous mold with further liquid mold material, and allowing recesses to form in the surface of the mold by the drawing-in of liquid mold material through shrinkage as the liquid mold material sets; placing tissue spheroids into the recesses in the surface of the porous mold; and sealing the tissue spheroids within the mold by topping off with liquid mold material and allowing the liquid mold material to set. An alternative method comprises the steps of: forming a mold of porous material from liquid mold material in a casting mold; allowing the liquid mold material to set; removing the porous mold from the casting mold; placing spheroids in recesses at the bases of wells in the mold of porous material; and sealing the spheroids within the porous mold by adding further porous material on top of the spheroids; wherein the recesses at the bases of the wells in the porous material are formed by protrusions of the casting mold carrying further, nipple-shaped, protrusions.

The present invention provides a cell detachment agent for detaching a cell to be separated from a separation carrier bound to the cell to be separated, wherein the above-mentioned cell detachment agent contains a bindable ureido group-containing polymer having a side chain structure represented by the following formula (1) and having a separation carrier-binding site capable of binding to the above-mentioned separation carrier at a side chain or terminal thereof, and the above-mentioned bindable ureido group-containing polymer has an upper critical solution temperature (UCST) of 4 to 40? C.:

NH(C?O)NH.sub.2(1)