Disclosed is a cell culture medium for cultivating cells, containing an iron-citrate-diphosphate complex as an iron source. Also disclosed is a method for cultivating cells, one or more cells being replicated or maintained in the cell culture medium and to a method for expressing at least one recombinant protein in a cell culture, a nucleic acid being introduced into the cell replicated or maintained in the cell culture medium, wherein the nucleic acid causes the production of at least one recombinant protein. The cell culture medium is advantageous in that the iron source contained therein dissolves very efficiently and rapidly in an aqueous solution, can be efficiently imported into the interior of the cells, causes an increased cell viability and an increased product titer during the production of recombinant proteins, and is very inexpensive.

The present invention is in the field of the cultivation of biological cells and tissues with organ-like function on a microphysiological scale and provides a method for the microphysiological co-cultivation of 3D organoid tissue and at least one 2D cell layer.

A cell fiber production system comprises: a first flow path through which a first fluid containing a cell flows; a second flow path for allowing a second fluid for preparing a hydrogel to converge around the first fluid in the first flow path; and an ejection port through which at least the first fluid and the second fluid are discharged together. The cell fiber production system is constructed that, at a start stage of production of a cell fiber, the first fluid is delivered after the second fluid reaches at least a convergence point between the first flow path and the second flow path.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

Disclosed is a poultry egg-based culture medium relating to the field of biotechnology, in particular a culture medium that can be used for cultivating mammalian cells, the culture medium containing a poultry egg content and a diluent. Also related is preparation and use of the culture medium, and a method of using the culture medium for cultivating mammalian cells.

The present disclosure is directed to RNA-regulated fusion proteins comprising a protein of interest and an RNA-regulated destabilization domain. Also disclosed are RNA aptamers that bind specifically to a RNA-regulated destabilization domain. Nucleic acid molecules encoding the RNA-regulated fusion proteins and RNA aptamers and methods of use thereof are also disclosed.

Disclosed herein is a recombinant human cell line that does not express functional endogenous NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (NDUFS2). This knockout cell line can be used to advance the basic understanding of the NDUFS2 protein subunit in overall complex I assembly and function. Furthermore, this cell line can be used to test therapeutic agents to fix or bypass the dysfunctional complex I.

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

This invention provides RNA, oligoribonucleotide, and polyribonucleotide molecules comprising pseudouridine or a modified nucleoside, gene therapy vectors comprising same, methods of synthesizing same, and methods for gene replacement, gene therapy, gene transcription silencing, and the delivery of therapeutic proteins to tissue in vivo, comprising the molecules. The present invention also provides methods of reducing the immunogenicity of RNA, oligoribonucleotide, and polyribonucleotide molecules.

Provided herein are cytoplasts, compositions comprising cytoplasts, methods of using cytoplasts, and methods of treating a subject, such as providing benefits to a healthy or unhealthy subject, or treating or diagnosing a disease or condition in a subject. In some embodiments, methods of treating a subject include: administering to the subject a therapeutically effective amount of a composition comprising a cytoplast. Also, provided herein are compositions (e.g., pharmaceutical compositions) that include a cytoplast. Also, provided herein are kits comprising instructions for using the compositions or methods.