Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.

The present invention provide a method of constructing a ligand-receptor protein complex to determine the binding activity of different PBDEs derivatives to an enoyl-ACP reductase. The method comprises providing a ligand-receptor binding complex, molecular docking and performing molecular dynamic simulation. The present invention is able to determine the binding activity of PBDEs derivatives to the enoyl-ACP reductase comparable to the results obtained in vitro.

The present invention relates to methods of producing Amyelois transitella pheromone precursors and genetically modified plants and microorganisms capable of producing Amyelois transitella pheromone precursors. The genetically modified plants and microorganisms include a heterologous gene encoding a first fatty-acyl desaturase and a second fatty-acyl desaturase in combination, and a fatty-acyl reductase.

The present invention relates to methods of producing Diatraea saccharalis pheromone precursors and genetically modified plants and microorganisms capable of producing Diatraea saccharalis pheromone precursors. The genetically modified plants and microorganisms include a heterologous gene encoding a first fatty-acyl desaturase, a second fatty-acyl desaturase, and a fatty-acyl reductase.

The present disclosure provides engineered enone reductase enzymes (EREDs), polypeptides having ERED activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing ERED enzymes are also provided. The present disclosure also provides engineered ketoreductase enzymes (KREDs), polypeptides having KRED activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing KRED enzymes are also provided. The present disclosure further provides compositions comprising the ERED and KRED enzymes and methods of using the engineered ERED and KRED enzymes. The present disclosure finds particular use in the production of pharmaceutical compounds.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

Provided is a method of producing at least one omega-hydroxyl fatty acid, the method comprising: (a) contacting at least one alkane with at least one recombinant yeast cell in an aqueous medium, wherein the yeast cell is capable of oxidising the alkane to the corresponding omega-hydroxyl fatty acid and the yeast cell comprises a reduced fatty acid degradation capacity.

The invention relates to a biocell (1) with a biofuel reservoir intended to be brought into contact with a liquid medium and with a fluid medium comprising an oxidant. Said biocell comprises a first electrochemical cell having: an anode (5) comprising a first enzyme capable of catalyzing the oxidation of the biofuel; —a cathode (7) comprising a second enzyme capable of catalyzing the reduction of the oxidant; and —a separating and porous membrane (3), electrically insulating, and permeable to said liquid medium, placed between the anode (5) and the cathode (7). Said biocell (1) being characterized in that it comprises a means for storing the biofuel (3) and for providing the liquid medium to the anode (5), said means comprising a hydrophilic porous material in contact with said anode (5)) and having a basis weight of 500 to 900 g/m2.

Provided herein are methods for identifying expression of SDHA, MIF, and or monosomy 3 or disomy 3 status in sample to identify the sample as high-risk melanoma and/or the sensitivity to oxidative phosphorylation inhibitors. Also provided herein are methods for treating monosomy 3 uveal melanoma by administering a SDHA inhibitor in combination with an oxidative phosphorylation inhibitor.

Provided is a microorganism that is able to produce 2-phenylethanol at a high concentration, and a method of efficiently producing 2-phenylethanol by using a saccharide as a raw material.

Provided is a coryneform bacterium transformant in which a shikimate pathway is activated, and further, a gene that encodes an enzyme having phenylpyruvate decarboxylase activity is introduced in such a manner that the gene can be expressed.

Also provided is a 2-phenylethanol producing method that includes causing the coryneform bacterium transformant according to the present disclosure to react in water containing a saccharide.