The present invention discloses a CLALS protein, its coding gene and use in predicting the herbicide resistance of a watermelon. The herbicide resistance of a watermelon to be tested in which the amino acid residue at position 190 from N-terminus of the CLALS protein is only a non-proline residue or a watermelon to be tested in which the amino acid residue at position 190 from N-terminus of the CLALS protein is a non-proline residue and a proline residue is stronger than that of a watermelon to be tested in which the amino acid residue at position 190 from N-terminus of the CLALS protein is only a proline residue. Experiments have shown that the type of the amino acid residue at position 190 from the N-terminus of CLALS protein can be used as a detection target to predict the herbicide resistance of a watermelon to be tested. The invention has great application value.

The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate (2H3M4OP) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M4OP, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO.sub.2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.

The herbicide-tolerant rice cultivar designated CL272 and its hybrids and derivatives are disclosed. CL272 is a novel, herbicide-resistant, early maturing, semidwarf, medium-grain rice cultivar with improved yield and improved disease resistance. This invention also pertains to methods for producing a hybrid or new variety by crossing the rice variety CL272 with another rice line, one or more times. This invention allows for single-gene converted plants of CL272. This invention also provides regenerable cells for use in tissue culture of rice plant CL272. The present invention provides a method for treating rice.

The herbicide-tolerant rice cultivar designated CL153 and its hybrids and derivatives are disclosed. CL153 is a novel, herbicide-resistant, high yielding, early maturing, semidwarf, long-grain rice cultivar with excellent grain yield, good grain quality, and produces plans with very good resistance to blast disease. This invention also pertains to methods for producing a hybrid or new variety by crossing the rice variety CL153 with another rice line, one or more times. This invention allows for single-gene converted plants of CL153. This invention also provides regenerable cells for use in tissue culture of rice plant CL153. The present invention provides a method for controlling weeds in the vicinity of rice.

Provided are a microorganism for producing a pantoic acid, and a construction method therefor and an application thereof. The microorganism for producing the pantoic acid is obtained by knocking out a gene in Escherichia coli and introducing an exogenous gene. The obtained microorganism is Escherichia coli that is registered in the China General Microbiological Culture Collection Center with an accession number of CGMCC No. 21699. A pantoic acid synthesis pathway has been opened up, and accumulation of the pantoic acid can be achieved in a fermentation process.

A mutant carotenoidogenic bacterium, comprising any of genes (a)-(c) below: (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; (b) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; and (c) both of the genes (a) and (b) above.

The present disclosure relates to a microorganism for producing a mycosporine-like amino acid, and a method for producing a mycosporine-like amino acid using the microorganism.

The microorganism of the present disclosure shows an improved ability for producing a mycosporine-like amino acid and thus can be effectively used in the production of a mycosporine-like amino acid.

Provided herein are non-naturally occurring microbial organisms comprising a methane-oxidizing metabolic pathway. The invention additionally comprises non-naturally occurring microbial organisms comprising pathways for the production of chemicals. The invention additionally provides methods for using said organisms for the production of chemicals.

Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing N additives to food, pharmaceutical, and nutritional supplement products.

Described herein are devices and methods for using the same to produce carotenoids. The carotenoids produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods. The devices and methods disclosed herein also enhance one or more physical properties of plants treated with the devices described herein.