The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.

A system and method for controlling metabolic enzymes or pathways in cells to produce a chemical above the levels of a wild-type strain is disclosed. The system utilizes cells, including yeasts, bacteria, and molds, having at least two genes capable of being controlled bi-directionally with light, where one gene is turned from off to on when exposed to light and another gene is turned from on to off when exposed to light, the two genes reversing when the light is turned off. Cells may utilize any number of sequences that benefit chemical production, including sequences that: encode for constitutive transcription of light-activated transcription factor fusions; encode for a metabolic enzyme; encode for a repressor; induce expression of metabolic enzymes; and an endogenous or exogenous activator expressed by a constitutive promoter, inducible promoter, or gene circuit. These systems may be coupled to biosensors or protein cascade systems, enabling the monitoring or automation of the fermentation process to optimize production of a desired product. These systems may also allow for optimization and periodic operation of a bioreactor using light pulses.

The present disclosure relates to the transformed Synechococcus elongatus strain of capable of mass production of farnesene. The transformed Synechococcus elongatus strain of the present disclosure is characterized by having the ability to mass produce farnesene using carbon dioxide as an independent carbon source. In particular, the Synechococcus elongatus strain is economically effective because it uses carbon dioxide present in light and air as a carbon source. There is an eco-friendly effect since it can be used for eliminating or reducing carbon dioxide in the atmosphere using microorganisms. Further, the strain of the present disclosure has a rapid growth rate and excellent ability to fix carbon dioxide compared with other microorganisms, thereby being utilized in various fields such as food, medicine, pharmacy, biofuel, and chemistry.

The present disclosure relates to nucleic acids that encode enzyme activities involved in the synthesis of lathyranes, intermediates in the synthesis of lathyranes and also compounds derived from lathyranes such as tiglianes, daphnanes and ingenanes; cells transformed with the nucleic acid molecules and vectors comprising the nucleic acid molecules.

This invention is aimed at improving an ethanol fermentation ability of a recombinant yeast strain having an ability of assimilating pentose, such as xylose or arabinose. The recombinant yeast strain haying an ability of assimilating pentose is obtained by lowering activity of a gene involved in upstream of glyceraldehyde-3-phosphate in the Embden-Meyerhof pathway.

The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.

A method for producing L-isoleucine at a higher yield by fermentation includes the step of using a genetic engineering method to obtain a genetically engineered strain. The genetically engineered strain has a threonine deaminase gene substantially releasing the inhibition of L-isoleucine and/or an acetylated hydroxy acid synthetase III gene substantially releasing the inhibition of L-isoleucine; and performing fermentation culture on the genetically engineered strain, adding diketobutyric acid or a raw material capable of being converted into diketobutyric acid in a culture process, and separating L-isoleucine from a culture after the end of culturing. Further provided is a genetically engineered strain for realizing high yield of L-isoleucine.

An engineered yeast strain capable of efficient fermentation of xylose to ethanol, and methods of making and using the strain, are provided.

The present disclosure relates to an acetohydroxy acid synthase variant in which the feedback inhibition to L-valine is released, a polynucleotide encoding the acetohydroxy acid synthase variant, an expression vector including the polynucleotide, a microorganism producing L-valine including the acetohydroxy acid synthase variant, and a method for producing L-valine using the microorganism.

Provided herein is D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase, and a construction method and use of the engineered strain. The D-lactate dehydrogenase has unique properties and is from Thermodesulfatator indicus, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and said gene engineering reconstruction method, a fermentation product of the reconstructed Bacillus licheniformis can be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state. By using the method for producing D-lactic acid through fermentation at high temperature, the production cost can be reduced, the production efficiency can be improved and there is a wide industrial application prospect for the method.