The present invention provides a recombinant bacterium and methods of using the recombinant bacterium to induce an immune response.

Described herein are methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.

The present invention relates to at least one cell for producing at least one lipid with general formula II from at least one carbon substrate, ##STR00001##
wherein R.sup.1 and R.sup.2 independently of one another comprises identical or different organic radicals each with 5 to 13 carbon atoms,
wherein the cell is a non-pathogenic cell that is genetically modified to increase the heterologous expression relative to the wild type cell of: an enzyme (E.sub.2) capable of converting 3-hydroxyalkanoyl-3-hydroxyalkanoyl-CoA/ACP or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) and NDP-glucose into β-D-glucopyranosyl-3-hydroxyalkanoyl-3-hydroxyalkanoate.

Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.

The invention relates to recombinant microorganisms and methods for producing mogroside compounds and mogroside precursors.

The present invention relates to engineered carbohydrate-active enzyme constructs that are useful for glycan polymers synthesis. The construct comprises a CD domain from a GH, wherein the domain is conjugated to CBM3a via a peptidic linker. The invention also relates to a method of improving glycan polymer synthesis by using engineered carbohydrate active enzymes comprising a CD domain and CBM3a.

Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.

Disclosed are components, systems, and methods for glycoprotein or recombinant glycoprotein protein synthesis in vitro and in vivo. In particular, the present invention relates to components, systems, and methods for identifying amino acid glycosylation tag motifs for N-glycosyltransferases and the use of the identified amino acid glycosylation tag motifs in methods for preparing glycoproteins and recombinant glycoproteins in vitro and in vivo.

Provided are a mutant glycosyltransferase UGT76G1 and a use therefor, the catalytic activity, the substrate selectivity, and/or the substrate specificity of the mutant glycosyltransferase UGT76G1 having been changed. Mutation at specific points can promote the catalytic activity for 1,3-glycosylation of a substrate containing 1,2-diglucosyl (sophorosyl), and weaken the catalytic activity thereof to perform 1,3-glycosylation on a glucose monosaccharide substrate base. Also provided are mutations that weaken the catalytic activity of glycosyltransferase UGT76G1, able to increase accumulation of a specific stevioside intermediate.

Polynucleotides encoding corresponding polypeptides capable of glycosylating steviol at its C-19 position to produce a steviol glycoside, an expression vector including such a polynucleotide, a method for producing a steviol glycoside by culturing a recombinant host cell containing such an expression vector under conditions in which the cell expresses the UDP-glycosyltransferase from the polynucleotide, and a method for producing a steviol glycoside by contacting a composition including steviol with a recombinant UDP-glycosyltransferase. The steviol glycoside can be steviol-19-O-glycoside. The recombinant host cell containing such an expression vector can be a bacterial cell, a plant cell, or a fungal cell, an animal cell, or a multicellular organism such as a plant.