A method for purification of a sulfatase using metal chelating chromatography without using tags such as His-tag, etc. is disclosed. An embodiment provides a method for purifying a sulfatase including the steps of: (a) providing a sulfatase-containing solution comprising one or a plurality of impurities; (b) performing a first chromatographic separation of the sulfatase-containing solution using a metal affinity chromatography resin; (c) performing a second chromatographic separation using a cation exchange chromatography resin; and (d) performing a final chromatographic separation using an anion exchange chromatography resin, wherein the impurities are removed thereby.

A method for purification of a sulfatase using metal chelating chromatography without using tags such as His-tag, etc. is disclosed. An embodiment provides a method for purifying a sulfatase including the steps of: (a) providing a sulfatase-containing solution comprising one or a plurality of impurities; (b) performing a first chromatographic separation of the sulfatase-containing solution using a metal affinity chromatography resin; (c) performing a second chromatographic separation using a cation exchange chromatography resin; and (d) performing a final chromatographic separation using an anion exchange chromatography resin, wherein the impurities are removed thereby.

A capsule composition comprising: (a) a polyester shell having a thickness of no more than 20 microns, and (b) a solution containing a visual and/or olfactory indicator, wherein the solution is encapsulated by the polyester shell. Also described herein is a method for detecting alpha particle radiation, in which: (i) the capsule composition is placed in contact with an esterase in a location where the presence of alpha particle radiation is being determined; (ii) waiting a period of time for the esterase to degrade the polyester shells, wherein the period of time is insufficient for the esterase to cause leakage of the solution in the absence of alpha particle radiation but is sufficient for alpha particle radiation, if present, to cause leakage from the capsule composition; and (iii) observing whether leakage has occurred at the end of the period of time to determine whether alpha particle radiation is present.

A capsule composition comprising: (a) a polyester shell having a thickness of no more than 20 microns, and (b) a solution containing a visual and/or olfactory indicator, wherein the solution is encapsulated by the polyester shell. Also described herein is a method for detecting alpha particle radiation, in which: (i) the capsule composition is placed in contact with an esterase in a location where the presence of alpha particle radiation is being determined; (ii) waiting a period of time for the esterase to degrade the polyester shells, wherein the period of time is insufficient for the esterase to cause leakage of the solution in the absence of alpha particle radiation but is sufficient for alpha particle radiation, if present, to cause leakage from the capsule composition; and (iii) observing whether leakage has occurred at the end of the period of time to determine whether alpha particle radiation is present.

The invention generally relates to methods of producing loline alkaloids or precursors thereof, expression constructs, and host cells useful for producing loline alkaloids or precursors thereof, and methods for producing loline alkaloids or precursors thereof in a host cell.

The invention generally relates to methods of producing loline alkaloids or precursors thereof, expression constructs, and host cells useful for producing loline alkaloids or precursors thereof, and methods for producing loline alkaloids or precursors thereof in a host cell.

This disclosure describes methods for regulating the biosynthesis of pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, 7-aminoheptanol, or 1,7-heptanediol by channeling increased flux through the biosynthesis pathway to obtain an intermediate required for growth of the host microorganism.

This disclosure describes methods for regulating the biosynthesis of pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, 7-aminoheptanol, or 1,7-heptanediol by channeling increased flux through the biosynthesis pathway to obtain an intermediate required for growth of the host microorganism.

The present invention relates to a brain tumor animal model that directly reflects the phenomenon in a human patient and a method of preparing the same, and more specifically, a brain tumor animal model that mutations are introduced into p53, Pten, and EGFR genes, a screening method of a therapeutic agent for a brain tumor using the animal model, and a preparing method thereof.

The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.