The invention provides a method for preparing an ordered cell-containing microarray, and a system for preparing an ordered cell-containing microarray.

The disclosure relates to an in situ-gelling hydrogel composition based on functionalized zwitterionic polymers. The resulting hydrogels exhibit highly anti-fouling, anti-adhesive, and lubricating properties to enable the fabrication of bulk hydrogels or hydrogel-based coatings of relevance to biomedical applications.

A living engineered glue system for performing autonomous mechanical repairs comprises a biofilm of microbial cells embedded in an extracellular matrix and operably linked in an environmentally-inducible, cell-cell communication genetic circuit to control gene expression.

A living engineered glue system for performing autonomous mechanical repairs comprises a biofilm of microbial cells embedded in an extracellular matrix and operably linked in an environmentally-inducible, cell-cell communication genetic circuit to control gene expression.

According to one embodiment, a microcapsule for selective catalysis of gases, the microcapsule comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In more embodiments, methods of forming such microcapsules include: emulsifying at least one biocatalyst in a polymer precursor mixture; emulsifying the polymer precursor mixture in an aqueous carrier solution; crosslinking one or more polymer precursors of the polymer precursor mixture to form a plurality of microcapsules each independently comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In further embodiments, corresponding methods of using the inventive microcapsules for catalyzing one or more target gases using include: exposing a plurality of the biocatalytic microcapsules to the one or more target gases.

According to one embodiment, a microcapsule for selective catalysis of gases, the microcapsule comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In more embodiments, methods of forming such microcapsules include: emulsifying at least one biocatalyst in a polymer precursor mixture; emulsifying the polymer precursor mixture in an aqueous carrier solution; crosslinking one or more polymer precursors of the polymer precursor mixture to form a plurality of microcapsules each independently comprising: a polymeric shell permeable to one or more target gases; and at least one biocatalyst disposed in an interior of the polymeric shell. In further embodiments, corresponding methods of using the inventive microcapsules for catalyzing one or more target gases using include: exposing a plurality of the biocatalytic microcapsules to the one or more target gases.

Proposed is a bioink supply system and, more particularly, proposed is a bioink supply system including: a hydrogel storage part; cell storage part; a mixing part configured to receive and mix a hydrogel and a cell solution from the hydrogel storage part and the cell storage part; a sensor part configured to measure a level of bioink inside a syringe; and a controller configured to receive a signal from the sensor part and maintain a constant level of the bioink inside the syringe, in which the mixing part supplies, to the syringe, the bioink prepared by mixing the hydrogel and the cell solution. The bioink supply system can continuously supply an active bioink to a syringe of a bioprinter during 3D bioprinting, and thus can continuously print large-scale biotissue, a plurality of organoids, organ-on-a-chip devices, etc.

Proposed is a bioink supply system and, more particularly, proposed is a bioink supply system including: a hydrogel storage part; cell storage part; a mixing part configured to receive and mix a hydrogel and a cell solution from the hydrogel storage part and the cell storage part; a sensor part configured to measure a level of bioink inside a syringe; and a controller configured to receive a signal from the sensor part and maintain a constant level of the bioink inside the syringe, in which the mixing part supplies, to the syringe, the bioink prepared by mixing the hydrogel and the cell solution. The bioink supply system can continuously supply an active bioink to a syringe of a bioprinter during 3D bioprinting, and thus can continuously print large-scale biotissue, a plurality of organoids, organ-on-a-chip devices, etc.

The present disclosure relates to microcapsules, methods of using such microcapsules in the delivery of drugs and probiotic microbes to subjects in need thereof, and methods of using such microcapsules for in vitro culture of microbes. In particular, the microcapsules comprise novel siloxane-based membranes that maintains transport properties essential to communication and growth of microbes.

Disclosed is a 3D porous medium and a method of manufacture. The 3D porous medium includes (i) a support structure of transparent hydrogel particles or emulsion droplets, (ii) bacterial nutrient in open volumes between the transparent hydrogel particles, as well as within micropores in the transparent hydrogel particles, and (iii) bacterial cells within the open volumes in the support structure.