Patent classifications
C12N11/10
METHOD AND APPARATUS FOR GENERATION OF MICROPARTICLES CONTAINING IMMOBILIZED ENZYME
The invention concerns a method and an apparatus for generation of micropartides containing an immobilized functional component, where the following measures are proposed: —spraying a liquid (32) containing a soluble alginate and a functional component consisting of molecules or nanoparticles to generate a stream (60) of droplets, —directing the stream (60) of droplets onto a precipitation bath (16) and capturing the droplets therein by application of high voltage (14), —precipitating the droplets in the precipitation bath (16) by means of a precipitation liquid (18) containing an alginate complexing agent, such that the droplets are solidified to form micropartides (10) containing the functional component and —extracting the micropartides (10) from the precipitation bath (16).
METHOD AND APPARATUS FOR GENERATION OF MICROPARTICLES CONTAINING IMMOBILIZED ENZYME
The invention concerns a method and an apparatus for generation of micropartides containing an immobilized functional component, where the following measures are proposed: —spraying a liquid (32) containing a soluble alginate and a functional component consisting of molecules or nanoparticles to generate a stream (60) of droplets, —directing the stream (60) of droplets onto a precipitation bath (16) and capturing the droplets therein by application of high voltage (14), —precipitating the droplets in the precipitation bath (16) by means of a precipitation liquid (18) containing an alginate complexing agent, such that the droplets are solidified to form micropartides (10) containing the functional component and —extracting the micropartides (10) from the precipitation bath (16).
Multidispensor cartesian robotic printer
Disclosed are systems, compositions, and methods for three-dimensional (3D) printing. An example system includes a plurality dispensers configured to deposit materials from their tips and a printing surface for receiving the materials. The system includes a position sensing detector configured to detect positions of the tips of the dispensers and the location and dimensions of the printing surface. The system includes a robotic positioning device configured to drive the dispensers. The system also includes a control unit configured to receive and map in a 3D space the positions of the tips of the dispensers and the position and dimensions of the printing surface. The control unit is further configured to control the robotic positioning device to drive the dispensers relative to the printing surface in the 3D space, and to independently deposit materials on the printing surface, or on material deposited on the printing surface.
COMPOSITION FOR DUST SUPPRESSION
The invention relates to an aqueous composition for the suppression of dust, comprising 1) an anionic surfactant; 2) an amphoteric surfactant; 3) a Bacillus species; and 4) a carbohydrate-based feedstock on which the Bacillus can grow. The composition is capable of forming a foam. When mixed with dust, the composition, in particular the foam thereof, is effective in preventing the dust from becoming airborne. Further, when applied before grinding or milling a material, the spreading of dust during and after the grinding or milling is effectively prevented.
COMPOSITION FOR DUST SUPPRESSION
The invention relates to an aqueous composition for the suppression of dust, comprising 1) an anionic surfactant; 2) an amphoteric surfactant; 3) a Bacillus species; and 4) a carbohydrate-based feedstock on which the Bacillus can grow. The composition is capable of forming a foam. When mixed with dust, the composition, in particular the foam thereof, is effective in preventing the dust from becoming airborne. Further, when applied before grinding or milling a material, the spreading of dust during and after the grinding or milling is effectively prevented.
Reversible surface functionalization
Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
Reversible surface functionalization
Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
HYDROGEL COMPOSITIONS COMPRISING ENCAPSULATED CELLS AND METHODS OF USE THEREOF
The present invention provides injectable compositions comprising cells encapsulated in hydrogel capsules and methods of preparing these compostions. The present invention also provides methods for using these compositions to promote hematopoiesis and to treat or prevent cardiovascular and immunological disorders in a subject.
HYDROGEL COMPOSITIONS COMPRISING ENCAPSULATED CELLS AND METHODS OF USE THEREOF
The present invention provides injectable compositions comprising cells encapsulated in hydrogel capsules and methods of preparing these compostions. The present invention also provides methods for using these compositions to promote hematopoiesis and to treat or prevent cardiovascular and immunological disorders in a subject.
Programmable and printable biofilms as engineered living materials
Three-dimensional, living, self-regenerative structures of predetermined geometry comprising solidified print material comprising a biofilm of Bacillus subtilis comprise a TasA-R protein, wherein R is a recombinant, heterologous functional group, wherein the TasA-R provides a preferably tunable physiochemical property like viscosity, reactivity, affinity as a function of the R group.