Provided herein is chitosan dually derivatized with arginine and gluconic acid; and methods of making and using the same, e.g., for gene delivery in vivo.

Provided herein is chitosan dually derivatized with arginine and gluconic acid; and methods of making and using the same, e.g., for gene delivery in vivo.

The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants.

The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants.

The present invention relates to the novel use of saponins having acetyl residues on one of their sugar residues. These saponins are able to enhance the transfection efficiency to a surprisingly much higher extent than already known saponins and even than Lipofectamin.

The present invention relates to the novel use of saponins having acetyl residues on one of their sugar residues. These saponins are able to enhance the transfection efficiency to a surprisingly much higher extent than already known saponins and even than Lipofectamin.

In one aspect, a structure for use in transfecting cells is disclosed, which comprises a matrix supporting a plurality of cavities, each cavity having an opening characterized by a rim and an inner surface subtending and/or extending from said rim. An electrically conductive coating is disposed on a top surface of the substrate between, and connecting, the rims of the cavities. A layer of an electrically conductive material can also coat at least a portion of each cavity's inner surface. At least one dimension of each cavity is in a range of about 50 nm to about 3.5 microns, e.g., in a range of about 100 nm to about 1 micron, or in a range of about 200 nm to about 800 nm, or in a range about 200 nm to about 500 nm. In some cases, all dimensions of the cavity (e.g., X, Y, an Z-Cartesian dimensions) are in the aforementioned ranges.

In one aspect, a structure for use in transfecting cells is disclosed, which comprises a matrix supporting a plurality of cavities, each cavity having an opening characterized by a rim and an inner surface subtending and/or extending from said rim. An electrically conductive coating is disposed on a top surface of the substrate between, and connecting, the rims of the cavities. A layer of an electrically conductive material can also coat at least a portion of each cavity's inner surface. At least one dimension of each cavity is in a range of about 50 nm to about 3.5 microns, e.g., in a range of about 100 nm to about 1 micron, or in a range of about 200 nm to about 800 nm, or in a range about 200 nm to about 500 nm. In some cases, all dimensions of the cavity (e.g., X, Y, an Z-Cartesian dimensions) are in the aforementioned ranges.

Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instauces, micellic assemblies provided herein are pH sensitive particles.

Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instauces, micellic assemblies provided herein are pH sensitive particles.