A method of coating a surface with nanoparticles for biological analysis of cells that includes the steps of cleaning the surface with an oxidizing acid, treating the surface with an organosilane, coating the surface with nanoparticles, and then growing cells on the surface coated with the nanoparticles. The surface may be a glass surface, a silica-based surface, a plastic-based surface or a polymer-based surface. The nanoparticles may be gold-based nanomaterials.

Methods for developing disease-related nanobodies and related products and kits are provided. The disease-specific proteins are extracellular matrix (ECM) proteins, domains or epitopes that are associated with various aspects of disease and are not present, or are present in very low quantities, in non-diseased individuals. Highly effective nanobodies capable of specifically binding to these ECM protein epitopes useful in in vivo imaging assays, the detection, diagnosis and treatment of diseases as well as monitoring therapeutic progress in a patient with a disease are provided herein.

A culture medium for testing drug resistance of H. pylori as well as a preparation method and use thereof are characterized in that 10% to 15% of calf serum, 1.2 mg/mL to 2.4 mg/mL of urea, 0.004 mg/mL to 0.016 mg/mL of phenol red, 10 ?mol/L to 100 ?mol/L of nickel chloride and H. pylori selective additive accounting for 1% of the total volume of the culture medium are added on the basis of a Columbia culture medium, and meanwhile, antibiotics for testing drug resistance are added, and the pH value is adjusted to 7.15 to 7.35. The present invention provides a method for a rapid testing of H. pylori resistance in culture media.

A bioelectronic device for regulating stem cell differentiation, a method for differentiating stem cells using the same, and a method for manufacturing the bioelectronic device. According to the present invention, it is possible to effectively control the differentiation of stem cells at a single-cell level, and to simultaneously perform a free radical inhibition function.

Disclosed are recombinant endoxylanases that are expressed and exported in high levels in bacteria, in particular Bacillus subtilis. The recombinant endoxylanases are based on an endoxylanase selected from Trichoderma resei or Bacillus pumilus modified with a signal peptide from B. subtilis alpha amylase (AmyE), B. subtilis levanase (SacC), or B. subtilis YwmC. Accordingly, also disclosed are plasmids for transforming bacteria to express and export such recombinant endoxylanases and the resulting recombinant bacterial strains. The disclosure also encompasses compositions for simultaneously degrading and assimilate cellobiose and xylan comprising a recombinant bacteria engineered to produce xylose from hydrolyzing the agricultural biomass and a xylose assimilator and methods of producing value-added products, for example succinate, from agricultural biomass using such compositions.

This invention relates to forming unique nanohybrid structures through selective integration of inorganic antimicrobial nanoparticles with other inorganic and organic materials and then, chemically bonding the nanohybrid structures to organic polymers for application as surface coatings, antimicrobial surfacing, food packaging, biomedical, agricultural, air-filtration/cleaning and water filtration/cleaning applications to kill, inhibit, and/or reduce the growth of pathogenic/infectious/contaminating microorganisms and their biofilms.

The present invention relates to a method of transporting a stem cell population, the method comprising transporting the stem cell population contacted with a liquid carrier. In addition, the present invention concerns a method of treating a subject having a disease, the method comprising topically administering a defined mesenchymal stem cell population to the subject, wherein the mesenchymal stem cell population is administered within about 96 hours from the time point the mesenchymal stem cell population has been harvested. Also concerned is a unit dosage comprising about 20 million cells, of about 15 million cells, of about 10 million cells, of about 5 million cells, of about 4 million cells, of about 3 million cells, of about 2 million cells, of about 1 million cells, of about 0.5 million cells, of about 0.25 million cells or of less than 0.25 million cells of a defined mesenchymal stem cell population.

The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more growth factors, one or more fatty acids, one or more buffer components, selenium and one or more further trace elements and its use in the culture of cancer stem cells, in particular tumorsphere culture of cancer stem cells.

Described herein is a method of treating a disorder, the method comprising: administering to a subject in need thereof a composition that contains a population of somatic stem cells that are 0.3-6.0 micrometers in size and CD9+, CD349+, CD133, CD90, CD34, SSEA1+, SSEA4+, CD13+, or Stro1+, wherein the disorder is hair loss, osteoporosis, or a damaged tissue.

The present disclosure relates to the technical field of culture materials for fungi and provides a solid medium for Coriolus versicolor, and a preparation method and use. Bran is used as a main component in the solid medium, and has advantages in economy and environmental protection, and provides high Coriolus versicolor growth rate and strong contamination resistance. The solid medium avoids the tendency to contamination of current media during an experimental process, and reduces culture cost. The solid medium provides higher biomass and stronger contamination resistance than a potato dextrose agar (PDA) medium.