A method of producing a cardiomyocyte population, the method comprising culturing a cell population containing cardiomyocytes seeded at a cell density of 0.1×10.sup.5 to 5.0×10.sup.5 cells/cm.sup.2 in a culture solution to proliferate the cardiomyocytes.

An object of the present invention is to provide a method of producing a cell preparation for a joint medical treatment, in which a cell proliferation ratio can be improved, an amount of serum required can be reduced, and karyotype abnormalities in a culture process can be suppressed, a method of culturing a mesenchymal stem cell, and a cell preparation for a joint medical treatment, which is produced by the above method. According to the present invention, there is provided a method of producing a cell preparation for a joint medical treatment, the method including culturing a mesenchymal stem cell in a culture medium containing an ascorbic acid-2-phosphate trisodium salt, alanyl glutamine, and pyridoxine.

Provided is a method for culturing immature oocytes. The method can promote in vitro maturation of the immature oocytes, and specifically comprises using follicular cells and a culture medium for culturing same. The culture medium for culturing the follicular cells contains CNP or variants thereof or analogues thereof and an HDAC (histone deacetylase) inhibitor. Also provided are the in vitro maturation culture medium containing CNP or variants thereof or analogues thereof and the HDAC inhibitor, and related compositions thereof, and the use of the above medium, culture medium and compositions in the promotion of in vitro maturation of the immature oocytes.

Provided are an exosome preparation formed by secretion by a mast cell cultured in vitro, a preparation method of an exosome therein, an exosome containing FcεRI protein on an outer surface thereof and in a substantially unbound state, and uses of the exosome preparation or exosome in a method for inhibiting mast cell activation in vitro and in preparing a drug for treating an IgE-mediated disease.

The disclosure discloses recombinant Bacillus subtilis for synthesizing e lacto-N-neotetraose yield. The recombinant Bacillus subtilis is obtained by integrating two β-1,4-galactotransferase genes on a genome of a host bacterium Bacillus subtilis 168ΔamyE:P.sub.43-lacY, P.sub.43-lgtB, P.sub.xylA-comK and exogenously expressing a β-1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinant Bacillus subtilis of the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation of Bacillus subtilis for producing the lacto-N-neotetraose.

The present disclosure provides methods for generating hematopoietic progenitor cells. In some embodiments, the methods involve an in vitro or ex vivo cell culture model utilizing retinoic acid signaling for producing hematopoietic progenitor cells from pluripotent stem cells.

The present disclosure relates generally to therapeutic agents that may be useful as inhibitors of Integrated Stress Response (ISR) pathway.

A synthetic meat product for human and animal consumption and methods for producing such food product are disclosed. The synthetic food product comprises or essentially consists of a cell biomass of avian cells grown in vitro in a chemically-defined serum free culture medium under controlled conditions and do not contain any hazard contaminations.

This invention relates to culture methods and media for in vitro expansion of hepatocytes, particularly primary hepatocytes; hepatocyte cultures and organoids obtainable and obtained by said methods; and uses of said hepatocyte cultures and organoids.

Cells derived from postpartum tissue and methods for their isolation and induction to differentiate to cells of a chondrogenic or osteogenic phenotype are provided by the invention. The invention further provides cultures and compositions of the postpartum-derived cells and products such as lysates related thereto. The postpartum-derived cells of the invention and products related thereto have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications, for example, in the treatment of bone and cartilage conditions such as osteoarthritis.