The present invention provides a novel culture medium and method for culturing stem cells, preferably muscle-derived mesenchymal stem cells. The culture medium comprises clotted blood plasma at 5-70% in base medium. The cultured cells may be released without use of trypsin by resolubilizing the clotted blood plasma with the assistance of proteolytic enzymes or anticoagulation agents.

There are provided compositions and methods for lyophilization and/or storage of live vaccine strains of Francisella tularensis. More specifically, there are provided lyophilization media and uses thereof for the preparation and long-term storage of Francisella tularensis vaccines.

Microorganisms and microbial production methods for the biosynthesis of ester compounds are provided. Useful examples employ microorganisms that have been genetically modified to express alcohol acyltransferases, either from other species or that have been modified to increase their activity in catalyzing the esterification of alcohols. Additional useful examples employ microorganisms that have been genetically modified to express lipases, either from other species or that have been modified to increase their activity in catalyzing the esterification of organic acids. Additional modifications are presented that significantly increase ester production.

The present invention relates to a method of isolating a mesenchymal stem cell population from the amniotic membrane of the umbilical cord, the method comprising cultivating umbilical cord tissue in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum). The invention also relates to a mesenchymal stem population isolated from the amniotic membrane of the umbilical cord, wherein at least about 90% or more cells of the stem cell population express each of the following markers: CD73, CD90 and CD105 and lack expression of the following markers: CD34, CD45 and HLA-DR. The invention also relates to a pharmaceutical composition of this mesenchymal stem population.

The present application relates to gelatin microparticles containing nutrients for cell culture therein, a preparation method therefor, and a use thereof. The gelatin microparticles of the present application can be advantageously used in cell cultivation for preparing cultured meat.

The present invention refers to a freeze-dried composition consisting of an isolated microorganism belonging to the Mycobacterium tuberculosis complex, preferably a M. tuberculosis clinical isolate, more preferably M. tuberculosis clinical isolate, characterized in that it comprises a PhoP− phenotype by the inactivation by a genetic deletion of the Rv0757 gene and the deletion of a second gene, Rv2930 (fadD26), that prevents PDIM production (PDIM− phenotype) (the MTBVAC strain), and sucrose and sodium glutamate as stabilizers or excipients. The present invention further refers to the reconstituted composition obtained by adding water, preferably sterilized water for injection, to the freeze-dried composition as well as uses thereof, in particular for use as a prophylactic agent to those at risk of infection with M. tuberculosis or those at risk of developing tuberculosis disease, or as secondary agents for treating infected tuberculosis patients.

Disclosed in the present disclosure are a hydroxybutyl chitin, a hydroxybutyl chitin hydrogel and a preparation method thereof. Chitin is subjected to pulverization, dissolution, modification with epoxybutane, and purification to obtain a final product, namely the hydroxybutyl chitin. The hydroxybutyl chitin prepared by the method has good solubility in purified water, and a hydrogel with a low solid content can be formed. The hydroxybutyl chitin hydrogel will have a wide application aspect in biomedicine, absorbable materials, and other fields.

Provided are thiol-acrylate hydrogels and tunable cell culture materials including thiol-acrylate hydrogels, and methods of making thereof. Also provided are systems for forming three-dimensional cell culture scaffolds including the materials, and methods of culturing cells, including cancer cells, using thiol-acrylate hydrogels and tunable cell culture materials. The materials herein can be used in microfluidic droplet-generating devices.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A medium supplement for high-yield culture of anaerobes is disclosed. The medium supplement includes N-acetylhexosamine, L-aspartic acid, L-cysteine and cobalamin. A culture medium including the supplement and a culture method using the culture medium are also disclosed. It is possible to provide an innovative method which is capable of achieving high-concentration culture of anaerobes that are difficult to culture in high yield. The method is cost-effective, and in particular, is capable of culturing large amounts of fastidious aerobes suitable for use in food and pharmaceutical applications.