Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.

A method of efficiently recovering a large amount of exosomes from mesenchymal stem cells is provided. The method includes: a three dimensional culture step of three dimensionally culturing mesenchymal stem cells in a medium containing sugar by using a nonwoven fabric as a scaffold; a post-plateau culture step of further culturing for a certain period of time after the amount of the sugar consumed by the mesenchymal stem cells reaches a plateau; and an exosome recovery step of recovering exosomes from the mesenchymal stem cells. The mesenchymal stem cells are adipose-derived mesenchymal stem cells.

A monocytes derived signaling cells mixture is prepared by culturing buffy coats in a cell culture medium containing platelet-poor-plasma and collecting the cultured buffy coats and the cultured cell culture medium. The monocytes derived signaling cells mixture contains granulocytes, lymphocytes, fibrocytes and monocytes, in which the high ratio of the monocytes are CD16 positive monocytes. The monocytes derived signaling cells mixture is used in the improvement of liver function index of a subject.

A system for generating and using an assay key comprising growth conditions for a set of microorganisms for a set of diverse QAC-based culture media under a variety of incubation conditions known to modulate the effect of QAC on growth of some microorganisms in the set. Each culture medium is characterized by a pH and includes one or more QACs and one or more growth supplements. The set of culture media includes media comprising various combinations of pH, QAC type, QAC concentration, growth supplement type, and growth supplement concentration. The assay key can be used to identify a microorganism by inoculating a variety of growth media within the key and incubating the inoculated media under conditions within the key and comparing the resulting pattern of microorganism growth across the media and conditions with growth patterns for various known microorganisms across the media and conditions that are within the key.

The present invention relates to a method for increasing the galactose content of a recombinant protein produced in mammalian cells, wherein during the cultivation of said cells the pH of the cell culture is changed and a composition comprising nucleosides, transition metal salts and/or sugars is fed.

Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.

This disclosure relates to endothelial and smooth muscle like vascular tissue produced from urine cells. In certain embodiments, the disclosure relates to methods of producing endothelial and smooth muscle like vascular tissue by exposing urine derived cells with ETV2 in a first growth media under conditions such that the cells are modified to form a pool of cells expressing increased levels of endothelium surface markers and thereafter exposing the pool of cells to a second growth media under conditions such that the cells are modified to form tissue containing cells expressing increased levels of smooth muscle surface markers in addition to the endothelium surface markers. In certain embodiments, the disclosure relates to using cells and tissues reported herein for the treatment of vascular, cardiac, and wound healing indications.

Use of a sugar selected from lactose, fructose and raffinose, for increasing serpin expression in Bifidobacterium longum strain CNCM I-2618.

This application provides improved cell culture media and cell culture methods comprising N-acetylcysteine. These improved cell culture media and cell culture methods increase cell viability, cellular growth rate and/or reduce cell doubling time of cholesterol auxotrophic cells, myeloma cells, and hybridoma cells.

The present disclosure relates to a production medium for microorganisms converting methanol to an organic acid and a culture process, wherein the converted organic acid is oxalic acid, and the production medium for microorganisms comprises 1 to 5% of methanol, 1 to 5% of xylose, and 0.01 to 0.05% of calcium chloride relative to 1 L of the total medium, and further comprises potassium dihydrogen phosphate (KH.sub.2PO.sub.4), ammonium sulfate ((NH.sub.4).sub.2SO.sub.4), magnesium sulfate (MgSO.sub.4), iron sulfate (FeSO.sub.4), manganese sulfate (MnSO.sub.4), zinc sulfate (ZnSO.sub.4), or boric acid (H.sub.3BO.sub.3). According to the present disclosure, provided is an organic acid production process using microorganisms of the genus Aspergillus (Aspergillus. sp), which enables a high-throughput production of high-value-added value organic acids such as oxalic acid by utilizing methanol obtained as a product from refining Cl gas such as methane.