Methods, kits, compositions, and systems are provided for culturing pluripotent stem cells to produce populations of cells comprising beta-like cells (e.g., pancreatic lineage, glucose-responsive, and/or insulin-producing). In particular, culture conditions are provided that result in the generation of beta-like cells from a starting culture of human pluripotent stem cells.

The present invention relates to a cell culture medium, in particular for culturing autologous fibroblasts, for use in aesthetic medicine for skin transplantation, said culture medium being serum-free and being characterized in that it contains glucose in a much lower quantity than conventional culture media.

The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.

Provided herein are methods of manufacturing β cells in vitro. Also provided herein are methods of treating a disease in a subject comprising administering the β cells manufactured in vitro to the subject. Also provided herein are methods of differentiating stem cells into β cells.

The present invention relates to media formulations free of swine serum and animal (primarily bovine brain and spinal cord) origin ingredients for Mycoplasma growth. Media formulations are rationally designed to preserve Mycoplasma antigenicity. Mycoplasma grown in these media formulations are useful in vaccines, particularly multivalent swine vaccines.

Methods for Generating Neural Crest-Like Cells and Nociceptor-Like Cells from Human Pluripotent Stem Cells are Provided Along with the Related Compositions.

An object of the present invention is to provide a medium with which hepatocytes can be cultured while a metabolic function thereof is maintained, a method of producing a hepatocyte using the above medium, and the hepatocyte that is obtained by the above method. According to the present invention, there is provided a medium for culturing a hepatocyte, containing one or more of a ROCK inhibitor, nicotinamide, and an analogous substance of the nicotinamide, in which a content of a serum component is less than 5% by volume, a content of an EGF receptor activating factor is less than 1 ng/mL, and a content of an ALK inhibitor is less than 0.1 μmol/L.

The invention provides pharmaceutical compositions comprising human immature dental pulp stem cells (hIDPSCs) wherein the hIDPSCs express CD44 and CD13. The invention also provides methods of treating a neurological disease or condition comprising systemically administering to a subject a pharmaceutical composition comprising hIDPSCs wherein the hIDPSCs express CD44 and CD13. For example, for treating neurological diseases or conditions including supporting the neuro-protective mechanism in subjects diagnosed with early HD or repairing lost DA neurons in subjects diagnosed with PD.

The present disclosure provides means of regenerating/restoring aged and/or damaged tissue or treating a medical condition by providing an extracorporeal circuit containing cells, including fibroblasts or dedifferentiated fibroblasts. that are in contact with plasma but not blood cells extracorporeally in a manner allowing for exchange of factors between a subject and an extracorporeally residing mass of cells. The disclosure provides means of titrating factors produced by the extracorporeal regenerative cell mass, thus allowing for a regenerative effect.

A method for promoting production of exosomes and/or extracellular vesicles is provided. The method includes culturing animal cells in a medium containing ascorbic acid, an analogue thereof, or a derivative thereof. The method is able to enhance the productivity of exosomes and/or extracellular vesicles secreted or released per cell or cell-derived conditioned medium.