The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The present invention provides a process for the treatment of sewage sludge with enzymes, which process comprises treating a sewage sludge resulting from the treatment of municipal or industrial waste water with a composition comprising a fermentation supernatant product from a Saccharomyces cerevisiae culture and a non-ionic surfactant, wherein said fermentation supernatant product is free of active enzymes, at conditions suitable for generating said active enzymes from said sewage sludge in situ.

The present disclosure relates to a composition for promoting the growth of lactic acid bacteria comprising growth factors, and more particularly, to a composition for promoting the growth of lactic acid bacteria comprising growth factors, the composition being capable of efficiently promoting the growth of various lactic acid bacteria strains.

A mixed bacteria for promoting nodulation and nitrogen fixation of Robinia pseudoacacia and their application are provided. The mixed bacteria includes Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28, all of which have been preserved in China Center for Type Culture Collection, and the preservation numbers respectively are: CCTCC No: M 2019237; CCTCC No: M 2019238; CCTCC No: M 2019239. The mixed bacteria are watered directly around the seedlings of Robinia pseudoacacia. Compared with the single bacteria control group and the sterile control group, the disclosure can produce synergistic superimposing effects, significantly improve the nodulation rate and symbiotic nitrogen fixation of the Robinia pseudoacacia, and promote the photosynthesis of the Robinia pseudoacacia.

The invention is directed to tools, compositions, and methods for the cultivation of microorganisms in culture media that is devoid of animal-derived materials such as blood, and, in particular, to compositions of meat-free media.

The present invention discloses an alkali-resistant microbial bacterium PDC-1 and its application. The present invention screens and isolates PDC-1 strain from the soil of a coal gangue stockpile in a coal mine in Shandong, China, which can utilize naphthalene, phenanthrene, anthracene or pyrene as the sole carbon source and has tolerance to heavy metals. The strain of the present invention is capable to degrade various PAHs with the presence of heavy metals. The strain is alkali resistant, simple to cultivate, and has a good application in the bioremediation of polycyclic aromatic hydrocarbons and heavy metal contaminated sites.

The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. TaXULne can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

The instant invention relates to the field of protein production, and in particular to compositions and processes for controlling and limiting the heterogeneity of proteins expressed in host cells.