The present invention relates to a medium composition for the dedifferentiation of induced pluripotency stem cells, containing a phlorotannin fraction extracted and isolated from one type of brown algae selected from the group consisting of Ecklonia cava, Dictyopteris prolifera, Dictyota coriacea, Sargassum horneri, Ishige okamurai and the like. In addition, the present invention relates to a method for preparing induced pluripotency stem cells by using the medium composition. Induced pluripotency stem cells can be safely, easily and effectively prepared by using mesenchymal stem cells by using the medium composition of the present invention, and the prepared induced pluripotency stem cells can be differentiated into various cells, and thus can be useful as a cell therapeutic agent.

Provided are a method for preparing a highly versatile aqueous solution having remarkably improved membrane filterability, which can be stably membrane-filtered in a short time, an aqueous solution prepared by the preparation method, a method for culturing cells using the aqueous solution which is prepared by the preparation method, a method for producing a physiologically active substance using the culturing method, a physiologically active substance produced by the method for producing a physiologically active substance, a method for performing membrane filtration of the aqueous solution which is prepared by the preparation method of the aqueous solution, a method for improving membrane filterability of the aqueous solution, and a method for producing the physiologically active substance by preparing the aqueous solution, performing membrane filtration of the aqueous solution, and then culturing cells using the resulting aqueous solution. The present invention relates to a method for preparing an aqueous solution, characterized by addition of a chelating agent.

The invention provides: a callus induction method that efficiently induces callus from a tissue fragment from an isoprenoid-producing plant; a callus culture method that efficiently grows callus; a somatic embryo induction method that efficiently forms a somatic embryo; a plant regeneration method that can stably regenerate callus into plants; a plant propagation method that can stably propagate a plant without being affected by e.g. weather and seasons; and a method of inducing rooting in a mature embryo. The present invention relates, inter alia, to a method of plant regeneration that can stably regenerate callus into plants and a method of plant propagation that can stably propagate a plant.

Provided is a culture material and a cultivation method of Ganoderma amboinense, which are used to increase the concentration of Ganoderiol F in the Ganoderma amboinense extract. First, the culture material is prepared, 3842 parts by weight of corn cobs, 6.657.35 parts by weight of wheat bran, 6.657.35 parts by weight of rice bran, 1921 parts by weight of seaweed powder, 23.7526.25 parts by weight of sawdust, and 0.951.05 parts by weight of sucrose are mixed evenly to obtain a fungi bag. Next, sterilization is performed, followed by inoculation of the fungi of Ganoderma amboinense into the fungi bag and subsequent cultivation. Finally, Ganoderma amboinense is harvested when the fruiting body of the Ganoderma amboinense grows to a stipe length of 20-25 cm, and spore powder has not been released.

Disclosed are methods for reducing contaminant levels in materials through remediation, including remediation using a filamentous fungus.

A serum-free culture media for expansion of the number of cells and related method of use are disclosed. The disclosed media can be for use in cultured food applications. The disclosed media can include a baseline serum-free and animal component-free culture media and a plant protein composition. The plant protein composition can comprise unhydrolyzed plant proteins. The plant protein composition can comprise plant proteins that are 3 kDa in size or greater. The plant protein composition can be present in the serum-free cell culture media at a final concentration of 0.05 g/L to 1 g/L. The baseline media, in use, provides a baseline growth capability for expansion of the numbers of muscle satellite cells for use in cultured food applications. The disclosed media, in use, provides an improved growth capability for expansion of the number of muscle satellite cells. Methods of making and using the disclosed media are also disclosed.

A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

The present invention provides a corn active peptide additive for cell culture medium, wherein in the corn active peptide additive, oligopeptides with molecular weight of lower than 1000 Dalton account for equal to or more than 90 wt % of total proteins, and the oligopeptides at least comprise one or more of AP, SAP, PAL, VNAP, PSSQ, and TQPGPQ. The corn active peptide additive of the present invention can be compounded with various basic culture mediums for serum-free culture of various animal cells, which not only substantially lowers the cost for cell culturing and reduces pollution and other problems caused by an animal derived component, but also can promote cell proliferation, improve cell viability and enhance expression of cell products.

Provided are non-naturally occurring nucleic acids comprising a sequence encoding an enzyme, regulatory protein, or other functional protein that provides for biosynthesis of monoterpene indole alkaloids (e.g., mitragynine), as well as modified enzyme and protein sequences encoded by the nucleic acid sequences. Also provided are recombinant microorganisms, including novel strains, that comprise the nucleic acid sequences and/or express the enzyme or regulatory protein that provides for the biosynthesis of monoterpene indole alkaloids (e.g., mitragynine). Methods of expressing the enzyme or regulatory protein are provided, as are methods of generating monoterpene indole alkaloid mitragynine-type compounds, as well as precursors, intermediates, and analogs thereof.

The present invention relates to an organoid and, more specifically, to an organoid and a use thereof, the organoid being produced using a carrier for cell culture which comprises microcapsules containing gelatin, a natural polymer, an oil, and an oil thickener. When used as a carrier for cell culture in culturing cells, the microcapsules containing a natural oil, according to the present invention, have the effects of improving adhesion and survival of the cells and inducing maturation of the cultured cells. The organoid produced by culturing cells using the carrier for cell culture has been confirmed to have the function of the organ concerned and, when treated with a drug, react to the toxicity of the drug and thus may be variously employed in the development of new drugs, disease research, and the field of artificial organ development.