The present invention provides a pigmentation skin model that comprises: a first cell group containing fibroblasts damaged by light irradiation, said first cell group being seeded on a first cell culture substratum; and a second cell group containing melanocytes and keratinocytes, said second cell group being applied onto the first cell group. The present invention also provides a method for producing the pigmentation skin model, and a method for evaluating a factor for treating or preventing pigmentation of the skin, said method comprising using the pigmentation skin model.

The present invention addresses the problem of providing: a culture medium that is for adherent culturing of pluripotent stem cells exhibiting high growth ability and that does not contain serum or albumin; and a highly versatile method for adherent culturing of pluripotent stem cells using the culture medium. According to the present invention, by adding, to a culture medium, a hydrophilic polymer that is conventionally known to inhibit adhesion of cells to the surface of a cell culture base material, so that the adhesion of pluripotent stem cell to the surface of the cell culture base material is enhanced, high growth ability is imparted in adherent culturing of pluripotent stem cells without adding serum or albumin thereto. In addition, by adding an antioxidant substance along with such a polymer to a culture medium, very high growth ability is imparted in adherent culturing of pluripotent stem cells.

A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.

We have discovered a chemical cocktail that selectively induces a robust expansion of myogenic stem cells from readily-obtainable dermal cells and from muscle stromal cells. By differential plating and lineage tracing, we show that Pax7+ cells were the major source for chemical-induced myogenic stem cells (CiMCs). We further performed single-cell RNA sequencing (scRNA-seq) analysis to characterize the transcriptomic profile of CiMCs and demonstrate a specific expansion of myogenic cells from heterogeneous dermal cell population. Upon transplantation into the injured muscle, CiMCs were efficiently engrafted and improved functional muscle regeneration in both adult and aged mice. Furthermore, an in situ therapeutic modality using this cocktail was developed by loading the chemical cocktail into injectable nanoparticles, which enabled a sustained release of the cocktail in injured muscle and a local expansion of resident satellite cells for muscle regeneration in adult and aged mice.

The present disclosure relates to the technical field of culture materials for fungi and provides a solid medium for Coriolus versicolor, and a preparation method and use. Bran is used as a main component in the solid medium, and has advantages in economy and environmental protection, and provides high Coriolus versicolor growth rate and strong contamination resistance. The solid medium avoids the tendency to contamination of current media during an experimental process, and reduces culture cost. The solid medium provides higher biomass and stronger contamination resistance than a potato dextrose agar (PDA) medium.

Methods for culturing cells, compositions comprising the cell culture product, and applications for the compositions are described. Methods include culturing cells with a media that includes a catalyst compound, which can include a stilbene or a nicotinamide compound, so as to control the secretome of the cells. Compositions that include the cell culture product include the cell secretome or components thereof in conjunction with at least one catalyst compound. Products can be utilized as a cell culture media or can be utilized in therapeutic applications.

Microorganisms and microbial production methods for the biosynthesis of ester compounds are provided. Useful examples employ microorganisms that have been genetically modified to express alcohol acyltransferases, either from other species or that have been modified to increase their activity in catalyzing the esterification of alcohols. Additional useful examples employ microorganisms that have been genetically modified to express lipases, either from other species or that have been modified to increase their activity in catalyzing the esterification of organic acids. Additional modifications are presented that significantly increase ester production.

A pomegranate molasses medium for culturing microbes includes a mixture of pomegranate molasses, soil extract, and water. The pomegranate molasses medium for culturing microbes may optionally include at least one of potassium dihydrogen phosphate and iron ammonium EDTA. At least one beneficial microbe may be cultured on the pomegranate molasses medium. The at least one beneficial microbe may include, e.g., Cyanothece and/or beneficial fungus.

A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.

Disclosed are a strain for producing nattokinase and a production method therefor. In particular, the present invention involves a novel strain capable of producing nattokinase, i.e. Bacillus subtilis natto ST-1086, deposited at the China General Microbiological Culture Collection Center under CGMCC No. 17895. The present invention further involves a method for producing a nattokinase product by means of using the novel strain CGMCC No. 17895 of the present application, wherein the resulting nattokinase product can be used as a drug for dissolving thrombi. The present invention further involves the use of the nattokinase product of the present application for preparing a composition for dissolving thrombi and in a method for treating thrombi.