Sperm mobility and impregnation of an oocyte is enhanced by placing sperm in an aqueous solution of phospholipids prior to in vitro fertilization or artificial insemination. The aqueous phospholipid solution can also be used during storage or cryopreservation of the sperm. Also, the motility of sperm produced or ejaculated and the environment into which it is placed is enhanced my ingestion of the phospholipid composition by the male or female, or a vaginal placement of compositions containing the phospholipids.

Disclosed is a L-isoleucine-producing Corynebacterium glutamicum fermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. Also disclosed is a method for cultivating the L-isoleucine-producing Corynebacterium glutamicum, comprising: inoculating the L-isoleucine-producing Corynebacterium glutamicum onto the fermentation medium, wherein the volume of the bacteria liquid accounts for 5-20% of the volume of the fermentation medium, adjusting the pH to 6.5-7 with aqueous ammonia, controlling the dissolved oxygen to 30-50%, and fermenting for 25-30 h; then decreasing the dissolved oxygen to 15-25%, and feeding a 50-80% glucose solution into the fermentation broth to control the residual sugar at 3-4%, continuing the fermentation until 60-70 hours, then terminating the fermentation, and controlling the temperature of the overall fermentation process at 29-33 C.

The present disclosure relates to cannabinoid compositions used in combination with stem cell therapies. These compositions can be encapsulated (e.g., microencapsulated). In particular, these compositions can be administered to a subject, such as through oral consumption or topical treatment.

The present disclosure discloses preparation of M. alpina CCFM698 thalli and application thereof in a feed additive, and belongs to the field of biological engineering and feed additives. The total fatty acid content of the M. alpina dried thalli obtained by the present disclosure is 30%-40% by weight of the dried thalli, and the EPA content is 24% or more by weight of total fatty acids. The dosage of the dried thalli in the feed in the disclosure is 0.5-1.5% of the total weight of the basal feed. The thallus feed additive is reasonable in fatty acid composition and very high in EPA content and can be used for producing high-DHA eggs which are beneficial to body health of eaters. The DHA content in each of eggs laid by laying hens fed with the feed containing the feed additive of the present disclosure reaches about 120 mg which is obviously higher than that in the prior art.

The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.

An object of the present invention is to provide an albumin-free medium for serum-free culture of pluripotent stem cells capable of maintaining the undifferentiated state and the pluripotency (pluripotent ability) of the pluripotent stem cells, an additive for an albumin-free medium, and a culture method. The albumin-free medium for serum-free culture of pluripotent stem cells, the additive for an albumin-free medium, and a medium used for the culture method of the invention are characterized by containing at least one selected from the group consisting of a Pluronic nonionic surfactant, an animal-based hydrolysate, and a nonanimal-based hydrolysate.

Provided is a culture medium composition including turmeric, glycine, or insulin for culturing muscle stem cells for proliferation of muscle stem cells. According to the culture medium composition including turmeric, glycine, or insulin muscle stem cells for culturing muscle stem cells according to an aspect, the proliferation and differentiation abilities of cells do not degrade even when culturing muscle stem cells in vitro, resulting in effects of enabling mass culture of muscle stem cells. Accordingly, the culture medium composition is economical by effectively replacing a culture medium containing expensive bFGF, and since appropriate time of differentiation can be chosen when culturing muscle stem cells, the muscle stem cells can be differentiated when needed, thereby producing an effect of improving the quality of in vitro meat.

The present disclosure relates to a cell culture composition including a Chlorella extract.

According to the cell culture medium composition including the Chlorella extract according to an aspect, the medium including the Chlorella extract can improve the cell proliferation ability of bovine myogenic stem cells. Thus, the Chlorella extract can be used as a substitute for fetal bovine serum.

The present invention relates to a composition for promoting the proliferation of stem cells derived from bone marrow using a palmultang extract, and more specifically, to a composition for promoting the proliferation of stem cells derived from bone marrow by administering a granulocyte colony-stimulating factor to a subject and then administering the palmultang extract to the subject. The composition of the present invention remarkably reduces side effects, such as enlargement of the spleen, which are caused by the administration of G-CSF alone for proliferation and differentiation of the stem cells, through administration in combination with the palmultang extract, thereby further promoting the proliferation and differentiation of stem cells.

A composition for treatment of tendonitis is provided. The composition comprises a pretreated adipose derived stem cell (ADSC), wherein the ADSC is pretreated with butylidenephthalide, and the concentration of butylidenephthalide is greater or equal to. The composition of the invention has abilities to repair damaged tendon fiber, enhance tissue regeneration, and decrease inflammation. The invention also provides a method for manufacturing a composition for treatment of tendonitis, comprising culturing an ADSC in a medium containing butylidenephthalide.