The present invention relates to a method for culturing a 3-dimensional lung cancer organoid and a method for preparing a patient-derived xenograft animal model using the same. More specifically, the present invention relates to a method for culturing a 3-dimensional lung cancer organoid, a lung cancer organoid prepared by the method, a medium composition for culturing the lung cancer organoid, a method for preparing a xenograft animal model using the lung cancer organoid, a patient-derived lung cancer organoid xenograft animal model prepared by the method, and a method for analyzing therapeutic efficacy of an anticancer agent and a method for screening an anticancer agent, using the animal model.

Disclosed herein are methods of expanding adherent stromal cells, including, inter alia, multi-step methods. Also disclosed are cells produced by the methods, which may be adherent stromal cells, for example placental adherent stromal cells. Further disclosed are pharmaceutical compositions comprising the cells. Additionally, methods of producing and utilizing the compositions, for example for therapeutic uses, are described.

Described herein are methods and compositions related to induced alveolar epithelial type 2 cells (iAEC2s), e.g., artificially-produced epithelial type 2 cells.

Disclosed cell therapeutics useful for regenerative, immune modulatory and angiogenic applications. In one embodiment the invention teaches uses of placentally derived cells possessing endothelial and mesenchymal features, said cells obtained by enriching for a subpopulation of cells in which said subpopulation expresses a CD45 negative phenotypic profile and further enriching for cells that express which express CD56. Said cells may be modified by culture in conditions that enhance regenerative, immunological, or angiogenic activities.

Provided is a cell culture medium comprising enriched basal media supplemented with ascorbic acid, a member of the fibroblast growth factor (FGF) superfamily, a transforming growth factor-beta (TGF-beta) superfamily ligand, and a stable glutamine source. Methods of culturing stem cells in such culture medium for extended periods of time, populations of stem cells, and kits are also provided.

Provided is a cell culture medium comprising enriched basal media supplemented with ascorbic acid, a member of the fibroblast growth factor (FGF) superfamily, a transforming growth factor-beta (TGF-beta) superfamily ligand, and a stable glutamine source. Methods of culturing stem cells in such culture medium for extended periods of time, populations of stem cells, and kits are also provided.

Described are means, methods and compositions of matter useful for enhancing quality of organ transplants by induction of postmortem regeneration. The disclosure provides administration of regenerative cells and/or factors in a brain dead recipient whose body is maintained in a viable state by life supporting machinery.

The technology relates in part to methods and compositions for ex vivo proliferation and expansion of epithelial cells.

The technology relates in part to methods and compositions for ex vivo proliferation and expansion of epithelial cells.

The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.