Disclosed herein are cell culture compositions, for example, pancreatic cell culture compositions, derived from dedifferentiated human reprogrammed pluripotent stem cells, such as induced pluripotent stem (iPS) cells, and methods for producing and using such cell culture compositions.

The present disclosure provides new and innovative compositions of Neuronal regeneration-promoting cells (NRPCs), methods for generating NRPCs, and methods of treating subjects having damaged nerve cells using NRPCs. In an embodiment, the NRPCs are induced from tonsil-derived mesenchymal stem cells and express CD26, CD106, CD112, CD121a, and CD141, wherein CD121a has an expression level of about 30% or higher, the expression level measured immediately after thawing the NRPCs from a frozen state. Furthermore, the NRPCs are configured to promote formation of axons on neuronal cells.

A culture medium for primary ovarian cancer cells, an in vitro culture method and an application thereof. The culture medium contains: an MST1/2 kinase inhibitor; a Rho kinase inhibitor, which is selected from at least one among Y27632, Fasudil and H-1152; an insulin-transferrin-selenium supplement; insulin; prostaglandin E2; an epidermal growth factor; gastrin; an insulin-like growth factor-1; cholera toxin; amphiregulin; N2; and B27. Compared with existing culture methods, the in vitro culture using said culture medium has higher amplification efficiency. Using the culture medium for the culturing of primary ovarian cancer cells may maintain the morphological structure and pathological features of primary tissues, and improve the success rate and survival rate of the cultured primary ovarian cancer cells.

Disclosed herein are cell culture compositions, for example, pancreatic cell culture compositions, derived from dedifferentiated human reprogrammed pluripotent stem cells, such as induced pluripotent stem (iPS) cells, and methods for producing and using such cell culture compositions.

A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

A composition is disclosed. The composition includes a cell culture medium; a proteinase; and a growth promoting factor. The doubling time of cells in the medium is the same or lower as compared to the doubling time of the cells in an otherwise identical composition which lacks the proteinase and has a higher concentration of the growth promoting factor. A method is also disclosed. The method includes growing the cells in a composition. The composition includes a cell culture medium; a proteinase; and a growth promoting factor. The doubling time of cells in the medium is the same or lower as compared to the doubling time of the cells in an otherwise identical composition which lacks the proteinase and has a higher concentration of the growth promoting factor.

The present invention relates to a medium composition for preparing an intestinal organoid. In addition, the present invention relates to: a method for preparing an intestinal organoid, comprising a step of culturing using the medium composition; and an intestinal organoid prepared by the preparation method. The medium for preparing an intestinal organoid can promote the proliferation of the intestinal organoid and maintain the stemness of the intestinal organoid. Therefore, the medium can be used in preparing a large amount of intestinal organoids of excellent quality, and intestinal organoids prepared using the medium can be used as agents for preventing or treating intestine-associated diseases, alternatives to animal experimental models of intestine-associated diseases, and the like.

The present invention relates to a medium composition for preparing a salivary gland organoid. Also, the present invention relates to: a method for preparing a salivary gland organoid, comprising a step of culturing salivary gland-derived cells in the medium composition; and a salivary gland organoid prepared by the preparation method. A medium for preparing a salivary gland organoid, according to the present invention, can promote the proliferation of a salivary gland organoid, maintain the stemness of the salivary gland organoid, and increase engraftment capability. Therefore, the medium can be used to prepare a large amount of salivary gland organoids with excellent quality, and salivary gland organoids prepared using the medium can be used as a preventive or therapeutic agent for salivary gland-related diseases, an alternative to animal experimental models for salivary gland-related diseases, or the like.