Methods using a soft polysaccharide hydrogel for an organoid culture are described. An example method includes preparing a cell suspension in a cell culture medium. The cells of the cell suspension embedded or cultured in the 3D cell culture biomatrix are injectable for in vivo application. The method also includes mixing a hydrogel solution with the cell suspension to form a soft hydrogel mixture, adding additional cell culture medium to the soft hydrogel mixture to obtain cell colonies after a first time period, harvesting the cell colonies, mixing the hydrogel solution with the cell colonies to create a 3D cell culture biomatrix, adding cell differential medium to the hydrogel solution, replacing the cell differential medium with an organoid transfer medium after a second time period, and replacing the organoid transfer medium with an organoid medium after a third time period.

The present invention comprises a test for the identification of an agent, which induces (re-)differentiation in a tumour cell based on the novel combined application of the two markers lactate as the end-product of the katabolic anaerobic glycolysis and neutral lipids as the end-product of the anabolic neutral lipid synthesis. The cell-based system is further characterized by a novel liquid handling procedure, which allows valid high throughput screening even over a longer time period of at least seven days, and a new viability test.

The present invention is directed to compositions and methods for the prevention or treatment of treatment of heterotopic ossification, vascular calcification, or pathologic calcification.

The present invention is directed to compositions and methods for the prevention or treatment of treatment of heterotopic ossification, vascular calcification, or pathologic calcification.

The present invention is directed to compositions and methods for the prevention or treatment of treatment of heterotopic ossification, vascular calcification, or pathologic calcification.

A collagen-fibronectin-based method enables the production of tumors of centimeter size with recognizable pathological traits. The method supports reproducing tumor heterogeneity with preinvasive and invasive phenotypes and stacking of tumor portions using a paper-scaffold with 80 ?m-punched holes for larger nodule creation and easy separation of tumor portions for analysis. Macrotumors are convenient for testing drug delivery and therapeutic tools that necessitate a minimum tumor size relevant to in vivo.

The present invention is directed to compositions and methods for the prevention or treatment of treatment of heterotopic ossification, vascular calcification, or pathologic calcification.

A method for producing mammary gland cells and a method of producing a mammalian milk like product, for example a human milk like product comprising generating lactocytes derived from mammalian induced pluripotent stem cells (miPSC), for example human induced pluripotent stem cells (hiPSC), and expressing the mammalian milk like product, for example the human milk like product from lactocytes.

Provided are methods for isolating subpopulations of stem cells. In some embodiments, the presently disclosed methods include selecting subsets of cells that are positive for CD34 or Sca-1, are further positive for one or more of FSHR, LHCGR, PRLR, AR, ESR, ESR, and PGR; and are negative for each of CD45R/B220, Gr-1, TCR, TCR, CD11b, and Ter-119. In some embodiments, the subpopulations are further fractioning into CD45.sup. and CD45.sup.+ fractions. Also provided are populations of stem cells isolated by the presently disclosed methods, compositions that include the presently disclosed subpopulations in pharmaceutically acceptable carriers, methods for expanding stem cells, methods for stimulating proliferation of MSCs, methods for treating subjects suffering from exposure to radiation, and methods for producing gametes in vitro.

Provided herein are cell constructs for producing a cultured milk product from mammary epithelial cells (MECs). In some embodiments, the cell constructs comprise a scaffold, a culture medium in fluidic contact with the scaffold, and mammary cells coupled to the scaffold. In some embodiments, one or more features and/or properties of the scaffold are specified so as to mimic a basement membrane to help to induce the secretory phenotype of mammary epithelial cells in vitro.