The invention relates to the use of a homeoprotein of the bicoid family, in particular of the Otx family, for enhancing the survival of cultivated retinal ganglion neurones, and for preventing or treating ganglion neuron degeneration particularly occurring in glaucoma.

An embodiment in accordance with the present invention includes 3D retinal tissue generated in a laboratory. The 3D retinal tissue is coupled to an engineered microelectronic chip. The 3D retinal tissue together with the engineered microelectronic chip enable retinal regeneration and vision restoration for patients with retinal cell damage. The engineered microelectronic chip sends electrical signals to specific parts of the 3D retinal tissue for stimulating and recording both the 3D retinal tissue and the cells in the patient's own retina. The chip may be absorbable or removable once connection is made between the 3D retinal tissue and the patient's own remaining retinal tissue.

The present invention provides ocular cells, genetically modified by a CRISPR system targeting the expression of B2M for ocular cell therapy. The invention further provides methods of generating an expanded population of genetically modified ocular cells, for example limbal stem cells (LSCs) or corneal endothelial cells (CECs), wherein the cells are expanded involving the use of a LATS inhibitor and the expression of B2M in the cells has been reduced or eliminated. The present invention also provides a cell populations, preparations, uses and methods of therapy comprising said cells.

Disclosed is a method for preparing an implant including a culture of cells on a membrane, the method including steps that consist of: securing the membrane to a mounting; placing the membrane in a recess of a housing providing two spaces having adjusted heights, above and below the membrane; injecting, into the spaces, a liquid capable of transforming into gel at a transport temperature lower than an injection temperature of the liquid; and bringing the housing to the transport temperature, so as to form an implant including the membrane and two layers of gel having adjusted thicknesses, on two opposite faces of the membrane, respectively.

The present application provides methods and processes for making and using a fibronectin composition, as well as methods for treating ocular conditions and/or disorders with the cellular fibronectin composition described herein.

The present invention relates to a population of amniocytes having phagocytic activity against pathological cells, such as tumour cells or the cells of other cellular diseases, such as, for example, proliferative vitreoretinopathy, the process for the preparation thereof and uses thereof in the medical field.

The present invention provides organotypic pig and non-human primate retina culture systems, and methods for evaluating or characterizing candidate therapeutic agents e.g. AAV-mediated gene vectors, using the same.

In some embodiments, the present invention provides tissue compositions including a first silk scaffold comprising a plurality of epithelial cells, a second silk scaffold comprising a plurality of stromal cells, and a plurality of neurons. In some embodiments, provided compositions can function as physiologically relevant corneal model systems for, inter alia, testing of therapeutics for corneal disease and/or injury and production of functional corneal tissue (e.g., for transplant, etc). The present invention also provides methods for making and using provided compositions.

The present invention provides a scaffold for culturing retinal tissue comprising an amount of gelatin, an amount of chondroitin sulfate, an amount of hyaluronic acid, wherein the amount of gelatin, chondroitin sulfate, and hyaluronic acid are prepared into a three-dimensional monolith, wherein the monolith is sectioned into planar sheets, and an amount of laminin-521.

The present invention discloses a method for culturing human limbal stem cells comprising the steps of: pre-treating a feeder cell; seeding the feeder cells in a porous membrane; placing the porous membrane in a container to separate the container into a first cell culture compartment and a second cell culture compartment, wherein the feeder cells are located in the second cell culture compartment; injecting the culture medium into the container; and placing the human limbal stem cells in the first cell culture compartment. Compared to the prior art, the method for culturing human limbal stem cells of the present invention utilizes the porous membrane to culture the human limbal stem cells to make the expansion rate better than the traditional culture method. In addition, the culture medium used in the present invention does not contain the dimethyl sulfoxide (DMSO) with cytotoxicity to reduce the probability of cell death.