Provided herein are methods and systems for co-differentiating differentiatable cells in a population of differentiatable cells into two or more different cell lineages. In some embodiments, the methods and systems use light- or chemically-activatable recombinases to drive expression of one or more transcription factors and/or differentiation factors.

The present application relates to methods for the generation of engineered human pluripotent stem cell-derived cardiac tissue, engineered cardiac tissue produced by such methods and methods for their use.