Provided are a method for producing a hepatocyte culture and a method for improving or maintaining a function of a hepatocyte or a hepatic progenitor cell, each method including a culturing step of culturing a hepatocyte or a hepatic progenitor cell inside at least a part of a plurality of opening pores and communication pores of a porous film having the plurality of opening pores provided on a surface thereof and the communication pores for communicating the opening pores adjacent to each other; and a cell-containing porous film.

Methods for producing hepatocytes from pluripotent human stem cells are disclosed herein. The stem cells are plated on a cell culture substrate comprising two laminins. The stem cells are then exposed to different cell culture mediums to induce differentiation. The resulting hepatocytes have higher metabolic capacity compared to hepatocytes cultured on different substrates.

The invention relates to the fields of biomaterials, tissue engineering and regenerative medicine. More specifically, it relates to biomaterial substrates having precise surface properties and the use thereof to investigate cell-material interactions. Provided is a cell culture system having a biomaterial substrate which has at least a first linear surface gradient oriented orthogonally to a second linear surface gradient, wherein the first gradient and the second gradient are selected from the group consisting of stiffness (S), (aligned) topography (T) and wettability (W). Also provided is a cell screening platform having a combination of at least two, preferably at least three, more preferably four distinct cell culture systems.

Provided are thiol-acrylate hydrogels and tunable cell culture materials including thiol-acrylate hydrogels, and methods of making thereof. Also provided are systems for forming three-dimensional cell culture scaffolds including the materials, and methods of culturing cells, including cancer cells, using thiol-acrylate hydrogels and tunable cell culture materials. The materials herein can be used in microfluidic droplet-generating devices.

Systems and methods for producing food products including cultured food products. The cultured food products include sushi-grade fish meat, fish surimi, foie gras, and other food types. Various cell types are utilized to produce the food products and can include muscle, fat, and/or liver cells. The cultured food products are grown in pathogen-free culture conditions without exposure to toxins and other undesirable chemicals. The food products can be processed to provide a desired shape, texture and consistency.

An artificial tissue construct for nerve repair and regeneration includes a biocompatible and biodegradable nerve guidance matrix comprising a plurality of biopolymers that include chitosan, gelatin, collagen and hyaluronic acid. A cross-linker includes glutaraldehyde. The nerve guidance matrix is formed as a three-dimensional scaffold polyelectrolyte complex (PEC). A subconfluent and grown monolayer of at least one of human mesenchymal stem cells, mesenchymal stem cells, differentiated Schwann cells and neuronal cells is on the biocompatible and biodegradable nerve guidance matrix for direct implantation or delivery. A method of making the artificial tissue construct is disclosed.

A three-dimensional cell culture scaffold and preparation method thereof. The preparation method comprises the following steps: designing, according to a shape of a cell culture device, a shape of the three-dimensional cell culture scaffold; printing once by a 3D printer to form the three-dimensional cell culture scaffold; performing a surface treatment, by a surface treating agent, on the three-dimensional cell culture scaffold manufactured by 3D printing to obtain a resultant three-dimensional cell culture scaffold. By adopting a three-dimensional printing technology to manufacture a three-dimensional fibrous cell culture scaffold having a three-dimensional fibrous network structure, raw materials and a manufacturing time can be saved, reducing production costs, increasing production efficiency, decreasing power consumption, preventing a flaw in the conventional manufacturing technique, and facilitating large-scale intelligent manufacturing.

The present invention relates to a nano-ligand for promoting cell adhesion and differentiation of stem cells and a method of promoting cell adhesion and differentiation of stem cells by using the nano-ligand, and the method of promoting cell adhesion and differentiation of stem cells according to the present invention may temporally and spatially, and reversibly control nano-ligand sliding by applying a magnetic field to a substrate including the nano-ligands, and efficiently control stem cell adhesion and differentiation ex vivo or in vivo through the magnetic-field based on spatiotemporal control.

Provided herein is a drug delivery device and composition, such as a particle, comprising conditioned medium. Also provided herein is a method of preparing polymeric particles for release of conditioned medium. Further, a tissue growth scaffold comprising particles for release of conditioned medium is provided.

The present invention relates to anticancer extracellular vesicles, a preparation method therefor, and an anticancer composition comprising same. Immune-tolerized extracellular vesicles containing LDHB and PGC-1α of the present invention provide cancer treatment, suppression of cancer metastasis, and cancer prevention technologies by normalizing cancer cell-specific aerobic glycolysis energy metabolic pathway in which lactate and hydrogen ions, which form a tumor microenvironment favorable for immune evasion, proliferation, metastasis and invasion of cancer cells, are produced, thereby enabling tumors to be effectively removed by means of the immune system of a patient.