A 3D cell culture gel kit and a 3D cell culture method using the same are provided. The 3D cell culture gel kit includes a gel material A, a buffer solution C, and a buffer solution D. The 3D cell culture method includes the steps of adding cells into a mixed solution containing the gel material A and setting the mixed solution at low temperature to get gel containing the cells. Then adding the buffer solution C to the gel for performing crosslinking. Next removing the buffer solution C and adding a growth medium. Let stand until the cells form spheroids in the gel. Moreover, the buffer solution D is used to dissolve the gel and the cells cultured are taken out for analysis. Thereby the 3D cell culture gel kit is convenient to use and suitable for 3D culture of a plurality of cell lines.

A three dimensional (3D) microfluidic cartridge suitable for reproduction of cells in the 3D microfluidic cartridge and for observing an angiogenesis process is provided. The 3D microfluidic cartridge includes a side area, wherein a height of the side area is greater than a height of central area. With the 3D microfluidic cartridge, a 3D cell culture is carried out, tumor spheroids are formed in the 3D microfluidic cartridge, and angiogenesis potentials of the tumor spheroids are measured. Further, responses of endothelial cells against angiogenic or antiangiogenic effects of various small molecules, drugs, and protein therapeutics are measured in the 3D microfluidic cartridge.

A method for producing a medium composition for suspension culture of an adherent cell, including the following steps: (i) a step of making an extracellular matrix carried on a nanofiber composed of water-insoluble polysaccharides, (ii) a step of adding the extracellular matrix-carrying nanofiber obtained in step (i) to a medium is provided by the present invention.

A method of qualifying whether a cell population is a suitable therapeutic for treating an eye condition is disclosed. The method comprises analyzing co-expression of premelanosome protein (PMEL17) and at least one polypeptide selected from the group consisting of cellular retinaldehyde binding protein (CRALBP), lecithin retinol acyltransferase (LRAT) and sex determining region Y-box 9 (SOX 9) in the population of cells.

The instant disclosure is directed to a method for vascularizing a pancreatic islet comprising culturing the pancreatic islet or β-cells with an endothelial cell comprising an exogenous nucleic acid encoding an ETV2 transcription factor under conditions wherein the endothelial cell expresses the ETV2 transcription factor. The instant disclosure is further directed to a method for making a vascularized β-cell organoid comprising culturing the pancreatic islet or β-cells with an endothelial cell comprising an exogenous nucleic acid encoding an ETV2 transcription factor under conditions wherein the endothelial cell expresses the ETV2 transcription factor. Disclosed also are vascularized islets and vascularized β-cell organoids produced by the methods of the instant disclosure, as well as methods for using the same.

A method of producing a three-dimensional cell structure includes producing a mixture of a cell cluster including an endothelial cell, an extracellular matrix component, and a polymer electrolyte, removing a liquid from the mixture to obtain a cell aggregate, and culturing the cell aggregate in a medium to obtain a three-dimensional cell structure with a thickness greater than 150 μm and having a vascular network. The extracellular matrix component is collagen or a collagen analog, and the polymer electrolyte is heparin or a heparin analog having a final concentration of 0.001 mg/mL or higher in the mixture.

The systems and methods of the present disclosure can be used to generate systems and models that are physiologically relevant to the human and animal system. These physiological conditions can be designed to mimic the actual human condition for cell differentiation and proliferation. The system and methods of this present disclosure allow the formation of an appropriate biomaterial to mimic that which exists in a human or animal scaffold. Utilizing 3D printing technology, a hydrogel scaffold can be printed at various resolution very close to human physiological geometry. Additionally, the architecture can be optimized for the selected application and appropriate cells can be seeded on the scaffold prior to testing.

The present invention provides a new method for producing Engineered Heart Muscle (EHM) under chemically fully defined conditions all compatible with GMP regulations. The resulting human myocardium generates force and shows typical heart muscle properties.

The disclosure relates to methods, systems and compositions for use in the production of milk. More specifically, the disclosure is directed to systems, compositions and methods for in-vitro production of milk using an array of mammary organoids seeded on tertiary-branched, resilient duct scaffolding.

Disclosed is a method of producing reconstructed human skin, and particularly a method of producing reconstructed human skin using a culture vessel including an inner chamber surrounded by an inner wall and a porous bottom surface and an outer chamber spaced apart from the inner chamber and configured to surround the inner chamber, including forming an adhesive layer by coating the inner wall with an adhesive material and culturing reconstructed human skin in the culture vessel having the adhesive layer formed thereon.