The present invention relates to a composition comprising mesenchymal stem cell-hydrogel-biodegradable support or mesenchymal stem cell-hydrogel-undegradable support, a sheet comprising the composition and a method for the preparation thereof. By using the sheet comprising the adipose-derived mesenchymal stem cell-hydrogel biodegradable or undegradable support, stem cells of high activity may be applied directly to the wound without isolation process using protease. The sheet has extracellular matrices such as collagen, laminin, fibronectin and elastin secreted by stem cells in the culture stage and included completely in the hydrogel, and therefore it has superior skin regeneration and wound healing effects compared with conventional pharmaceutical preparations and shortens therapeutic period.

The present invention provides a medical analysis device and a cell analysis method, which can capture many types of cancer cells, including cancer cells not expressing EpCAM. The present invention relates to a medical analysis device having a well portion, the well portion having a hydrophilic polymer layer formed at least partly on the inner surface thereof, the hydrophilic polymer layer having fibronectin adsorbed thereto.

There is provided systems and methods for performing fluidic perfusion, recirculation and interacting organ in standard wells or microfluidic reactors loading cells or organs into an insert or chip. The perfusion system can provide new media to the cell or organs while the circulation system can provide convective mixing of fluids within a well or between one or more organs in an assay. The system can be placed in an incubator or microscope and perform multimodal stimulation and sensing. The system includes electromechanical control, microfluidic lid and inserts or chips for performing automated cell based assay, organ of a chip or human on a chip in a remote-controlled environment.

Cell-culture platforms are described that can impart cyclic strain on a biologically-relevant culture substrate. Accordingly, systems and methods of the invention can provide a more accurate model of physiological strain and forces than earlier technologies. Systems of the invention enable high-throughput experimentation in standard culturing equipment, while utilizing a 3D physiologically-relevant environment.

The present disclosure relates to cultured adipose tissue. In one embodiment, the cultured adipose tissue is produced by culturing adipose cells in a culture media in vitro, harvesting the adipose cells after a desired amount of adipose cells are produced, and aggregating the harvested adipose cells to provide the cultured adipose tissue. In some embodiments, aggregating the harvested adipose cells comprises mixing the harvested adipose cells with a hydrogel or binder in a three-dimensional (3D) mold. In other embodiments, aggregating the harvested adipose cells comprises cross-linking the harvested adipose cells in a 3D mold. The cultured adipose tissue have a defined 3D shape and a size on the macroscale. In some embodiments, the cultured adipose tissue may be a food product.

The present invention relates to a mold for preparing a hollow 3D cell tissue structure such as an organoid, and uses thereof. Methods for preparing a hollow 3D cell tissue structure such as an organoid, in particular a human heart mimic, are also provided.

A therapeutic composition comprising a purified fraction of adipose-derived mesenchymal stem cells encapsulated in a three-dimensional biocompatible gel matrix, and methods, and systems for preparing and using encapsulated adipose-derived mesenchymal stem cells. Hydrogel microbeads encapsulating stem cells maintain the viability and location of the stem cells for an extended period as compared to stem cells in suspension. The gel matrix allows the release of cellular factors from the encapsulated stem cells to surrounding tissues to achieve desired therapeutic results.

Described herein are a system, device, methods and compositions related to generating 3-dimensional cardiac tissues. Also described herein are a system, device, and methods of maturing 3-dimensional cardiac tissues and maintaining their viability in culture.

The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.

The invention relates to a method for producing a material for preparing a three-dimensional, gel-like substrate, wherein a thrombocyte concentrate is provided and blood platelets are harvested from this thrombocyte concentrate. The blood platelets are then lysed so as to produce a platelet lysate, and said platelet lysate is subsequently lyophilized so as to provide a dry platelet lysate gel material. The invention further concerns a platelet lysate gel material comprising blood platelet lysate, wherein this material is a lyophilized dry material. The lyophilized gel can be easily reconstituted by the addition of water at the place of use in order to provide a completely functional platelet lysate-gel which can be used for cultivating living cells without any limitations. Accordingly, this method facilitates transportation of the gel material and ensures a higher shelf life of the platelet lysate gel without any impact on cell culture properties. Cells grown on such lyophilized hPL-gel show similar cell proliferation and expansion rates and no effect of the lyophilization on the differentiation potential and the immunophenotype profile of seeded MSCs compared to the fresh prepared hPL-gel without lyophilization can be observed.