A method of making a cardiac construct is carried out by depositing a mixture comprising live mammalian cardiac cells (e.g., individual cells, organoids, or spheroids), fibrinogen, gelatin, and water on a support to form an intermediate cardiac construct; optionally co-depositing a structural support material (e.g., polycaprolactone) with the mixture in a configuration that supports the intermediate construct; and then contacting thrombin to the construct in an amount effective to cross-link the fibrinogen and produce a cardiac construct comprised of live cardiac cells that together spontaneously beat in a fibrin hydrogel. Constructs made and methods of using the same are also described.

A hybrid gel comprising a particulate decellularized tissue (obtained by pulverizing animal-derived biological tissues that are decellularized (decellularized biological tissues)), fibrinogen and thrombin; a cell culture material comprising the hybrid gel; a method for preparing the hybrid gel; and a kit comprising a particulate decellularized tissue and a biological tissue adhesive are provided. The hybrid gel of the present invention exerts the effect to promote differentiation and gain of function of stem cells and the therapeutic effect to a variety of diseases.

A gel precursor composition causative of no apprehension about exhibiting cytotoxicity can be made. A method for producing a gel causative of no apprehension about exhibiting cytotoxicity, using the gel precursor composition can be performed. One of the present embodiments is a gel precursor composition including: a biocompatible polymer; a crosslinking agent to be activated by a specific substance; and a photoresponsive compound bound to the specific substance, or encompassing the specific substance.

Described herein are two-layer 3-D cultures, including those with microfeatures at the boundary between the two layers, as well as methods of making and using such cultures.

The present disclosure generally relates to an improved cell-culture growth medium, for example, for the production of cell-based meat, and other cultivated products, or applications. For example, certain embodiments are directed to a cell culture growth factor supplement comprising platelet lysate (PL) and platelet rich plasma (PRP). Such solutions may be used, for example, to increase the cellular biomass in a bioreactor by enhancing the rate and frequency of cellular proliferation. In some embodiments, platelet-rich plasma is isolated from the whole blood of a living animal. The platelets within the platelet-rich plasma may be concentrated, for example, by centrifugation, to produce a platelet concentrate. In some embodiments, the platelet concentrate can be cultivated and activated using an agonist to release growth factors. In some embodiments, the growth factor solution can be separated from the platelets and added to a bioreactor containing a cell-based meat product.

A tissue graft for soft tissue repair or reconstruction comprising a sheet of a biopolymer-based matrix having a plurality of small perforations and a plurality of large perforations. The small perforations are sized to facilitate clotting and granulation tissue development within the perforations which, in turn, facilitates revascularization and cell repopulation in the patient. The large perforations are sized to reduce the occurrence of clotting and granulation tissue development within the perforations so that extravascular tissue fluids accumulating at the implant site can drain through the tissue graft. The large perforations enhance mammal tissue anchoring by permitting mammal tissue to compress into the perforations increasing mammal tissue contact area.

A therapeutic composition comprising a purified fraction of adipose-derived mesenchymal stem cells encapsulated in a three-dimensional biocompatible gel matrix, and methods, and systems for preparing and using encapsulated adipose-derived mesenchymal stem cells. Hydrogel microbeads encapsulating stem cells maintain the viability and location of the stem cells for an extended period as compared to stem cells in suspension. The gel matrix allows the release of cellular factors from the encapsulated stem cells to surrounding tissues to achieve desired therapeutic results.

Various systems and methods of producing animal derived products, such as for cultivated meat, are described herein. For example, one embodiment includes the steps of obtaining components of a cell-culture medium by sustainably harvesting blood components from living animals to produce a cell-culture medium suitable for in vitro cell culture and culturing cells in the cell-culture medium to produce a cultivated meat product. In some embodiments, the disclosure generally relates to sustainably harvesting blood components for the cultivation of meat and other animal derived products, e.g., by repeatedly extracting blood components in a manner which does not kill a non-human animal. In some embodiments, the non-human animals can be farmed for the purpose of sustainably extracting whole blood and/or blood products. In some embodiments, the harvested blood product may be used to produce a cultivated meat product or other animal derived product, such as a muscle tissue, a fat tissue or heme.

The disclosure relates to a method for the differentiation of stem cells to endothelial cells, vascular smooth muscle cells, fibroblasts and keratinocytes; their use in the production of a organotypic vascularized skin or mucosa model or composition; a method relating thereto; the use of the model or composition in the testing of pharmaceutical and/or cosmetic agents; and including therapeutic and cosmetic skin compositions developed or tested thereby.

A method of incorporating cells in a porous matrix, the method comprising incorporating the cells in the porous matrix by forming a gel comprising the cells in situ in the porous matrix from a gellable medium, wherein the formation of the gel from the gellable medium comprises a time period of 10 minutes or less after contact of the gellable medium with the cells, thereby incorporating the cells in the porous matrix. The incorporated cells can then be used, for example, to produce a cultured skin product or deliver specific cells to a subject in need thereof.