The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.

A 3D-printed in vitro model biological microenvironment in examples discussed below may have one or more of the following features: (a) a gel matrix 3D-printed scaffold, wherein the gel matrix comprises a chemical composition configured to culture a first type of live cells, (b) a target chemical disposed at one or more locations within the gel matrix, the target chemical forming a chemical depot from which a chemical gradient is created within the gel matrix, (c) a conduit disposed within the gel matrix and defining a lumen comprising a second type of live cells, wherein the conduit is configured to enable at least some of the first type of live cells to migrate through the conduit and facilitate flow of at least: some of the live cells to an outlet of the conduit, or enable introduction of at least one of other cells, Achemical mediators, or drugs into the 3D-printed microenvironment.

Described herein are methods of generating a programmable multicellular organoid and/or a 3D organ-specific tissue. Also, described are the programmable multicellular organoid and/or a 3D organ-specific tissue produced by the described methods. Also, described herein are in vitro methods of generating functional human tissue construct.

A cellularized patch for treating a scar or skin condition of a subject The cellularized patch and methods of use thereof are advantageous in skin graft procedures performed, for example, to treat a subject having a skin condition comprising skin hypopigmentation or depigmentation, such as vitiligo. In some cases, human melanocytes are cultured ex vivo and seeded onto a transfer patch before being applied to a subject.

An object of the present invention is to provide a complex containing two or more neural retina-containing cell aggregates, and a method for manufacturing the same. The complex of the present invention is a complex comprising: two or more cell aggregates each containing a neural retina derived from a pluripotent stem cell; and a matrix, the two or more cell aggregates being arranged in the matrix. The method for manufacturing the complex of the present invention is a method for manufacturing a complex in which two or more neural retina-containing cell aggregates are arranged in a matrix, comprising: (1) a first step of preparing two or more cell aggregates of neural retinas from a pluripotent stem cell; and (2) a second step of contacting the two or more cell aggregates in a predetermined arrangement with the matrix or a precursor of the matrix, followed by the gelation of the matrix.

The present invention relates to providing artificial tendon or ligament tissue having sufficient strength. More specifically, artificial tendon or ligament tissue having sufficient strength is provided by embedding collagen-secreting cells in a gel having strength capable of resisting a tensile load and by culturing the cells while applying a tensile load to the gel to produce artificial tendon or ligament tissue. Cells that steadily express the Mkx gene can be used as the collagen-secreting cells. A fibrin gel containing aprotinin can be used as the gel.

A method of producing a three-dimensional cell tissue include obtaining a mixture including cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, gelling the mixture such that a gel composition including the cells, cationic substance, extracellular matrix component and polyelectrolyte is obtained; and incubating the gel composition such that a three-dimensional cell tissue is obtained.

This document provides methods and materials for performing retinal pigment epithelium transplantation. For example, methods and materials for using fibrin supports for retinal pigment epithelium transplantation are provided.

The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

A therapeutic composition comprising a purified fraction of adipose-derived mesenchymal stem cells encapsulated in a three-dimensional biocompatible gel matrix, and methods, and systems for preparing and using encapsulated adipose-derived mesenchymal stem cells. Hydrogel microbeads encapsulating stem cells maintain the viability and location of the stem cells for an extended period as compared to stem cells in suspension. The gel matrix allows the release of cellular factors from the encapsulated stem cells to surrounding tissues to achieve desired therapeutic results.