The present disclosure relates to a human bone marrow model. The present disclosure further relates to a microphysiological device comprising two or more side channels having endothelial cells therein and at least one central channel arranged therebetween, the at least one central channel having a cellularized scaffold formed therein, the cellularized scaffold of the at least one central channel including hematopoietic stem cells, mesenchymal stromal cells, and endothelial cells. In an embodiment, the device further comprises vasculature developed within and between the two or more side channels and the at least one central channel, wherein the vasculature developed within and between the two or more side channels and the at least one central channel includes anastomoses formed between vessels within the at least one central channel and an endothelium formed within the two or more side channels, the anastomoses permitting perfusion between the two or more side channels.

The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and/or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics.

A composition which is a hydrogel including, in an aqueous medium: at least one hydrophilic thickening polymer, and particles of eggshell membrane, in an amount of less than 5% by weight of the total weight of the composition, wherein the particles are rod-shaped, needle-shaped or fibrous, having a specific particle size. Also described is a bioink including the composition, a method for manufacturing a 2D or 3D product using the hydrogel, a kit including the hydrogel or bioink, and to uses thereof.

Frozen, porous thin films and methods of making and use thereof are described herein. The frozen, porous thin films can comprise a continuous phase permeated by a plurality of pores; wherein the continuous phase comprises a polymer, a ceramic material, or a combination thereof. In some examples, the frozen, porous thin films are made by freeze casting.

The present disclose relates to a high-reactivity hydrogel particle and preparation method therefor and use thereof. The preparation method comprises the following steps: (1) performing polymerization reaction of double-bond modified polyethylene glycol, a modified natural polymer and an optional active functional monomer to obtain a gel; (2) smashing the gel to obtain a gel particle; and (3) performing enzymolysis reaction of the gel particle and an enzyme to obtain the hydrogel particle with reactivity. The double-bond modified polyethylene glycol has a double bond and a polyethylene glycol chain segment, the modified natural polymer has sulfydryl or a double bond, the active functional monomer is selected from a sulfydryl functionalized biological adhesive peptide or sulfydryl functionalized cholesterol, and the enzyme is an enzyme corresponding to the natural polymer. The obtained hydrogel particle has high reactivity and can be used for subsequent secondary crosslinking. The hydrogel particle also has good biocompatibility.