The bioink disclosed herein includes one or more cells, a carrier material, and microspheres. The microspheres can include one or more biodegradable polymers and one or more compounds, such as a morphogenic compound. The methods disclosed herein can include three-dimensional bioprinting. Additional methods disclosed herein include producing functional tissue.

A process of preparing buccal epithelial cell suspension and cystoscopically implanting the cell suspension in the defect site of the adult human urethra.

Described are methods for producing tissue constructs, tissue constructs produced by the methods, and their use. The described method of producing a tissue construct comprises providing a granular tissue, depositing one or more filaments on or in the granular tissue, each filament comprising an ink, and gelling or fusing the granular tissue, thereby producing the tissue construct.

The present invention relates to a process for in vitro spermatogenesis from male germinal tissue comprising conducting maturation of testicular tissue comprising germ cells in a bioreactor which is made of a biomaterial and comprises at least one cavity wherein the germinal tissue is placed, and recovering elongated spermatids and/or spermatozoa.

The present invention is a cell transfer agent comprising a composite particle coated by a sugar chain polymer wherein the composite particle consisting of an apatite comprising phosphate, carbonic acid, and calcium.

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Disclosed herein are methods of producing a three-dimensional cell culture microenvironment, which may include exposing a lyophilized polycation coupon to a polyanion and a fluid medium. The lyophilized polycation coupon may optionally include a void volume, and the polyanion may optionally be contained within a slurry. Such methods may also include the steps of evaporating off the fluid medium of the slurry to produce a dried three-dimensional architecture, administering a hydrating fluid to the dried three-dimensional architecture, and producing the three-dimensional cell culture microenvironment. Also disclosed herein are three-dimensional cell culture microenvironments formed by various processes, as well as kits which include such three-dimensional cell culture microenvironments provided in a multi-well plate, and a hydrating fluid.

Provided are methods of controlling disassociation of cells from a carrier, compositions, and methods of collecting cells. The methods of controlling disassociation of cells from a carrier may include contacting a polymeric carrier with one or more digesting agents to disassociate at least a portion of a plurality of cells from the polymeric carrier. The polymeric carrier may be crosslinked with a crosslinker including at least one of a redox sensitive moiety, a UV light sensitive moiety, a pH sensitive moiety, and a temperature sensitive moiety.

The present disclosure provides a method of preparing mimicking angiogenic co-spheroids, including: co-cultring a neural related cell and a cultured cell on hyaluronan-grafted chitosan (CS-HA) substrates to form a co-spheroid of neural related cell/cultured cell, and encapsulating the co-spheroid of neural related cell/cultured cell into a hydrogel to form a mimicking angiogenic co-spheroid. The mimicking angiogenic co-spheroid of the present disclosure can be formed by 3D printing model as a 3D mini-neurovascular unit, which is applicated to a high-throughput angiogenesis screening platform.

The present invention provides a cell carrier containing a nanofiber composed of water-insoluble polysaccharides, preferably chitin or chitosan nanofiber; a carrier capable of being a common carrier in various operations such as i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, v) recovery of bioactive substance from culture supernatant and the like of adherent cells; and continuous performance of plural operations selected from i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, and v) recovery of bioactive substance from culture supernatant of adherent cells by using the carrier.