A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

The present invention provides compositions and methods for treating cancer.

Provided are methods of controlling disassociation of cells from a carrier, compositions, and methods of collecting cells. The methods of controlling disassociation of cells from a carrier may include contacting a polymeric carrier with one or more digesting agents to disassociate at least a portion of a plurality of cells from the polymeric carrier. The polymeric carrier may be crosslinked with a crosslinker including at least one of a redox sensitive moiety, a UV light sensitive moiety, a pH sensitive moiety, and a temperature sensitive moiety.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.

Tissue-engineered nerve scaffolds, articles for up-selecting desired cell proliferation and down-selecting undesired cell proliferation, and methods of manufacturing the same are provided. The tissue-engineered nerve scaffold includes a hydrogel having a surface. The surface has a topography including a micropattern defined by a plurality of spaced features attached to or projected into the hydrogel. The micropattern facilitates attachment and alignment of neural cells and reduces attachment and alignment of cells associated with scar-tissue formation and encapsulation.

The invention relates to medicine and biology, particularly to the means for artificial manufacturing of biological tissues and organs, and can be used in biotechnology, bioengineering, tissue engineering, regenerative medicine, and in the 3D-printing of biological tissues and organs.

Technical character of the invention consists in the development of a method of printing living tissues and organs as well as of the apparatus for its implementation. The proposed apparatus consists of at least: a printing platform, a bioink printing module with at least one nozzle designed for bioink dosing, a gel-forming composition printing module, containing a UV-module, and at least one nozzle capable of dosing gel-forming composition that starts polymerizing under the influence of UV radiation, a module for relatively displacing the nozzles and/or the platform,

and in which the bioink printing module is separated from the gel-forming-composition printing module in such a way so as to prevent UV radiation from reaching the bioink printing module, the radiation from the UV module being directed predominantly parallel to the platform for printing, in such a way so as to prevent UV radiation from reaching the biological tissues and/or organs being printed.

The technical result of the invention is the development of a multi-functional device capable of combining various printing modes, providing a method of high-resolution printing of living tissues and organs based on UV-induced hydrogel polymerization, and a method of cell protection from UV radiation during the printing process.

The present invention is directed to the use of microfluidics in the preparation of cells and compositions for therapeutic uses.

Provided herein are methods and compositions for the production of hepatocytes from placenta stem cells. Further provided herein is the use of such hepatocytes in the treatment of, and intervention in, for example, trauma, inflammation, and degenerative disorders of the liver. Also provided herein are compositions and methods relating to combinations of nanofibrous scaffolds and adherent placental stem cells and methods of using the same in cartilage repair. Finally, provided herein are compositions and methods relating to nonadherent, CD34.sup.+CD45.sup. stem cells from placenta.

A bioreactor and methods for use can include a microfibrous scaffold, that can be made of a composite bioink, and that can have endothelial cells directly embedded within the scaffold using an additive manufacturing process. The scaffold can further be seeded with cardiomyocytes. The hydrogel scaffold can be composed of a plurality of serpentine layers, with each serpentine layers, which can be placed on each other in a cross-hatch configuration, so that the primary axes of successive layers are perpendicular. This configuration can establish an aspect ratio for the scaffold, which can be selectively varied. For greater strength, the successive layers that have a primary axis in the same direction can be placed in the scaffold so that they are slight offset from each other. The scaffold can be placed in the bioreactor with perfusion, for use in cardiovascular drug screening and other nanomedicine endeavors.

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4 C. to about 80 C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).