Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.

Embodiments of the invention describe a cultured meat or food product with more desirable flavor and texture, as well as methods and compositions for producing the same. Certain embodiments are directed to methods of producing a stiffer, structured surfaces or scaffolds that mimic the extracellular matrix (ECM) and support the growth of myotubes that are interspersed with a scaffold component supporting fat cells (adipocytes) in vitro.

The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and/or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics.

A cell culture article, including: a substrate comprising a polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid having a degree of esterification from 1 to 40 mol %, or salts thereof; and an adhesion polymer on the surface of the polygalacturonic acid compound. A method of making and using the article are also disclosed.

Aspects of the present disclosure provide methods and compositions for immune cell activation. Disclosed are antibody-coated microparticles and methods for use. In some cases, immune cell activation methods comprising mechanical stimulation are disclosed. Embodiments are directed to activation of cytotoxic T cells. Additional aspects include generation and activation of regulatory T cells.

A method of maintaining cellular viability of harvested bone, where the method includes: providing a source of bone or bone particles; combining the bone or the bone particles with a sterile solution; and storing the bone or the bone particles in the sterile solution until their introduction into a patient.

Algae-containing beads comprising a microorganism such as unicellular microalgae, an insoluble carbon source such as char, water, and a crosslinked organic matrix. The beads can further contain clay, such as kaolin.

The invention relates to a process for producing carrier particles for the cultivation of biological cells, comprising the steps of providing an aqueous suspension of hydrogel beads and freeze-drying of the hydrogel beads so that dry hydrogel particles are formed, wherein at least one lyoprotectant substance is added to the suspension, the hydrogel beads are loaded in the suspension with the lyoprotectant substance and the dry hydrogel particles receive a shape under the effect of the lyoprotectant substance, which shape approximates a spherical particle shape and is still maintained after rehydrating. The invention also relates to carrier particles for a cultivation of biological cells, which comprise dry hydrogel particles, preferably having a protein coating, and to applications of the carrier particles.

Hydrogel beads having tunable rates of loading and unloading of cryoprotective agents are provided herein. Such hydrogel beads can be dispersed throughout a cell suspension to enable loading and unloading of cryoprotective agents from cells in a gradual and distributed manner that protects the cells from osmotic damage. Lymphocyte viability after cryopreservation is significantly greater when cryoprotective agents are loaded and unloaded using hydrogel beads compared to conventional media exchange methods.

A bioreactor and methods for use can include a microfibrous scaffold, that can be made of a composite bioink, and that can have endothelial cells directly embedded within the scaffold using an additive manufacturing process. The scaffold can further be seeded with cardiomyocytes. The hydrogel scaffold can be composed of a plurality of serpentine layers, with each serpentine layers, which can be placed on each other in a cross-hatch configuration, so that the primary axes of successive layers are perpendicular. This configuration can establish an aspect ratio for the scaffold, which can be selectively varied. For greater strength, the successive layers that have a primary axis in the same direction can be placed in the scaffold so that they are slight offset from each other. The scaffold can be placed in the bioreactor with perfusion, for use in cardiovascular drug screening and other nanomedicine endeavors.