Provided herein is a composition comprising a hydrogel having a shear modulus of about 20 Pa to about 1600 Pa conjugated with anti-CD3 antibodies and anti-CD28 antibodies. Also provided are methods of activating T cells and methods of killing cancer cells using the hydrogel composition.

The present disclosure relates to a nanofiber structure for cell culture, a method for manufacturing the structure, and a cell analysis device including the nanofiber structure for cell culture. The structure includes a cell culture layer made of nanofibers; and a spacer protruding upward from a surface of the cell culture layer, wherein the spacer divides a region on the cell culture layer into at least two culturing regions, wherein the spacer is made of the same nanofibers as the cell culture layer and thus has a cell migration channel defined therein.

The present disclosure relates to a method for producing cultured meat, the method including: forming a cell sheet by culturing cells usable for producing cultured meat; and culturing the cells in a form in which a nanofilm is formed on a surface of the cell sheet by coating the cell sheet. The present disclosure provides a method for producing cultured meat that implements excellent mechanical strength by protecting a cell layer from external stress and performing stable cell proliferation, and provides cultured meat implementing quality and taste that are improved compared to those of conventional cultured meat by reproducing tissue similar to muscle tissue of an actual animal.

Early vascular cells (EVCs), including endothelial cells and pericytes, are generated from hiPSCs. Unlike the isolated endothelial progenitor cells, the differentiated ECs mature and are functional. When encapsulated in synthetic hydrogel, EVCs respond to matrix cues and self-assembled to form three-dimensional EVCs. Moreover, these EVCs respond to hypoxic microenvironment and undergo vasculogenesis to form complex 3D networks.

The present disclosure relates to a hydrogel comprising hyaluronic acid modified by serotonin, a use thereof, and a method of preparing the same.

A method to prepare synthetic hydrogels having tissue-specific properties, and a hydrogel comprising a polymer matrix comprising a plurality of peptide, are provided.

A method or apparatus for creating a three-dimensional tissue construct of a desired shape for repair or replacement of a portion of an organism. The method may comprise injecting at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The apparatus may comprise an injector configured to inject at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The first material may comprise a yield stress material, which may be a material exhibiting Herschel-Bulkley behavior. The tissue construct may have a smallest feature size of ten micrometers or less.

Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.

In various aspects and embodiments, the present invention provides methods for preparing innervated tissue. In various embodiments the invention further provides innervated tissue generated using the methods described herein. In various embodiments the inclusion of optogenetically transducible TENGs or Micro-TENNs in the innervated tissue allows the modulation of tissue or organs by using light to stimulate the optogenetically transducible TENGs or Micro-TENNs.

An object of the present invention is to provide a vehicle and a composition for transplantation comprising retinal tissue and the vehicle, which are for the treatment of retinal degenerative diseases such as retinitis pigmentosa (RP) and are suitable for the subretinal transplantation of retinal tissue. The vehicle for transplantation of the present invention is a vehicle for transplantation for subretinally transplanting retinal tissue, the vehicle having a viscosity of 5 to 500 mPa.Math.s at a shear rate of 2 (1/s) at 25° C., and comprising hyaluronic acid and a pharmaceutically acceptable aqueous liquid. The composition for transplantation of the present invention comprises transplant retinal tissue and the vehicle for transplantation of the present invention.