The disclosure provides 3D bioprinted test beds and methods of making the 3D bioprinted teste beds, methods of using the 3D bioprinted test beds for testing and/or comparatively testing two or more test compounds on cell growth and/or behavior, as well as biocompatible methacrylated hyaluronic acid-based bioinks for printing the 3D test beds and/or other articles. The 3D test beds and bioinks include a hydrogel material/precursor and can include extracellular matrix components.

A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments.

Provided herein, in some embodiments, are human patient-derived brain tumor models and methods of use.

The present invention is to improve dynamic characteristics while maintaining temperature responsiveness in a cell culture support containing hydroxyalkyl chitosan, and to provide a cell culture support having temperature responsiveness, which contains temperature-responsive hydroxyalkyl chitosan and a water-soluble polymer selected from polyethylene glycol, derivatives thereof, hyaluronic acids, alginic acids and salts thereof.

This disclosure describes methods for organoid generation including, for example, for generation of a multi-tissue organoid. The multi-tissue organoid may include cartilage, bone, epithelium, and/or fibrous connective tissue. This disclosure further describes methods for isolating cells from the organoids and methods of using the organoids and cells of the organoids.

Disclosed are hydrogel compositions comprising both polysaccharide-based and polyzwitterionic components, methods of making the compositions, and methods of using the compositions for various clinical and biomedical applications.

A method or apparatus for creating a three-dimensional tissue construct of a desired shape for repair or replacement of a portion of an organism. The method may comprise injecting at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The apparatus may comprise an injector configured to inject at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The first material may comprise a yield stress material, which may be a material exhibiting Herschel-Bulkley behavior. The tissue construct may have a smallest feature size of ten micrometers or less.

The present disclosure provides compositions and methods for providing a cell replacement therapy to treat various diseases, including pancreatic diseases and diabetes. Specifically, the disclosure provides three-dimensional (3D) cell clusters of transdifferentiated insulin producing cells attached to scaffolds, such as a polysaccharide matrix, in order to provide a cell replacement therapy.

An object of the present invention is to provide a method for producing a sheet-like cell structure having excellent strength and shape-maintaining performance, and a sheet-like cell structure having excellent strength and shape-maintaining performance. According to the present invention, there is provided a method for producing a sheet-like cell structure, including: a step of adding a biocompatible macromolecular block, a cell, and a liquid medium onto a culture support body having a plurality of recessed portions on a culture surface, and immersing the biocompatible macromolecular block and the cell in uppermost portions of the recessed portions; and a step of culturing the cell to obtain a sheet-like cell structure.

Disclosed are cell culture scaffolds comprising a scaffold composition comprising chitosan and alginate, hyaluronic acid, or chondroitin sulfate, wherein the scaffold is formed from an aqueous solution comprising greater than 4 wt % chitosan. These scaffolds can be contacted with cells, e.g., patient-derived cancer cells. The scaffolds provide 3D structure allowing appropriate cell function to occur. A scaffold of chitosan and chondroitin sulfate are also disclosed. Scaffold compositions comprising chitosan and alginate, hyaluronic acid, or chondroitin sulfate are also disclosed. Methods of making these scaffolds, e.g., by freeze casting or 3D printing, and using them to evaluate a patient's cancer cell and personalize treatment are also disclosed.