Disclosed are genetically modified nudiviruses capable of being sexually transmitted by an insect useful for controlling pest populations. The genetically modified nudiviruses are capable of causing sterility in a target population of insects. Also disclosed are insects infected with the disclosed genetically modified nudiviruses, methods of making the genetically modified nudiviruses, and methods of using the genetically modified nudiviruses to control an insect pest population.

The present invention relates to the field of biotechnology, and discloses a hepatitis B virus mutant, a mutant amplification kit and use thereof. The HBV mutant of the invention takes place mutation of the hepatitis B virus mutant in genome sequence No. 216 and/or 285, the mutation occurred at position 216 is that base T mutates to C, the mutation occurred at position 285 is that base G mutates to A. The two mutations are closely related to the inflammation aggravation of the hepatitis B. The invention further provides a HBV mutant amplification kit to detect the above mutation, the usage of the kit and its application, therefore offering help for the clinical early diagnosis and intervention of severe hepatitis B, and providing references for further studying the functional changes caused by HBV gene mutation.

This invention relates to compositions, methods, strategies, and treatment modalities related to the epigenetic modification of hepatitis B virus (HBV) genes.

Disclosed are the H. zea nudivirus sequence, two additional related sequences from H. virescens and H. armigera nudiviruses for development of genetically modified nudiviruses capable of being sexually transmitted by an insect useful for controlling pest populations, methods for preparing egg populations for controlling pest populations and methods for controlling pest populations utilizing such egg populations. Such genetically modified nudiviruses are capable of causing sterility in a target population of insects. Previously disclosed were insects infected with the previously disclosed genetically modified H. zea nudivirus, methods of making genetically modified nudiviruses, and methods of using genetically modified nudiviruses to control an insect pest population.